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Human Neurturin Antibody

Catalog #: MAB387
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB387
MAB387-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neurturin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neurturin in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Neurturin is observed and no cross-reactivity with recombinant mouse (rm) Artemin, recombinant human (rh) Cripto-1, recombinant Drosophila DPP, rhGDNF, rhLefty A, rmLefty-1, rhMIS, rmNodal, rmPersephin, rhTGF-beta 1, rhLatent TGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2 or rhTGF-beta 3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 126422
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Neurturin
Ala96-Val197
Accession # Q99748
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Neurturin (Catalog # 1297-NE)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.15 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Neurturin (Catalog # 1297-NE) to immobilized Recombinant Human GFR alpha -2 Fc Chimera (Catalog # 613‑FR) coated at 1 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin is a member of the GDNF family of ligands and is distantly related to TGF-beta family ligands. It is a disulfide-linked homodimer that interacts with a receptor complex composed of a high affinity ligand binding component (GFR alpha 1 or GFR alpha 2) and a common signaling component, cRET. Neurturin promotes the survival of a variety of neurons and possibly other cell types.

Entrez Gene IDs
4902 (Human); 18188 (Mouse)
Alternate Names
Neurturin; NRTN; NTN

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Citation for Human Neurturin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structure and biophysical characterisation of the human full-length Neurturin-GFRa2 complex - a role for heparan sulfate in signalling
    Authors: J Sandmark, G Dahl, L Öster, B Xu, P Johansson, T Akerud, A Aagaard, P Davidsson, JM Bigalke, M Sörhede-Wi, GJ Rainey, RG Roth
    J. Biol. Chem., 2018-02-02;0(0):.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Development (Detection)

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