Human NFATC1 Antibody
Human NFATC1 Antibody Summary
Ala48-Ser406
Accession # O95644
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human NFATC1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC1 at approximately 110 - 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human NFATC1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for NFATC1 at approximately 124 kDa (as indicated) using 10 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NFATC1
NFATC1 (Nuclear factor of activated T cells c1; also NFAT2a) is a 120 kDa member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Human NFATC1 is 943 amino acids (aa) in length. It contains a calcineurin-binding site (aa 118‑123), two transactivation domains (aa 126‑218 and 703‑943), two NLSs (aa 265‑267 and 682‑684), an NES (aa 310-321) and an RHD that binds DNA (aa 410‑592). There are multiple isoforms. Individually or in common, there is an alternate start site at Met473, a deletion of aa 826‑943, a 29 aa substitution for aa 1‑42, and a 19 aa substitution for aa 698‑943.
Product Datasheets
Citation for Human NFATC1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Human venous valve disease caused by mutations in FOXC2 and GJC2
Authors: Oliver Lyons, Prakash Saha, Christopher Seet, Adam Kuchta, Andrew Arnold, Steven Grover et al.
Journal of Experimental Medicine
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