Human NGL-3/LRRC4B Antibody

Catalog #: MAB6919 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6919
MAB6919-SP

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Human NGL-3/LRRC4B Antibody Summary

Species Reactivity

Human

Specificity

Detects human NGL-3/LRRC4B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LRRC3, 4, or 32 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 702021

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NGL-3/LRRC4B
Ala47-Lys576
Accession # Q9NT99

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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NGL‑3/LRRC4B in Human Brain. NGL-3/LRRC4B was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human NGL-3/ LRRC4B Monoclonal Antibody (Catalog # MAB6919) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGL-3/LRRC4B

NGL-3 (netrin-G ligand-3), also called LRRC4B (Leu-rich repeat/LRR-containing glycoprotein 4B), is a 72 kDa (predicted) post-synaptic adhesion molecule of the LRR protein family. Mature human LRRC4B is a 678 amino acid (aa) type I transmembrane protein with a 541 aa extracellular region (aa 36-576) containing nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. Over the region used as an immunogen, human LRRC4B shares 97% aa sequence identity with mouse and rat LRRC4B. LRRC4B also shares ~55% aa identity with LRRC4C/NGL-1 and LRRC4/NGL-2, but each recognizes different ligands. LRRC4B recognition of the receptor tyrosine phosphatases LAR/PTPRF, PTPR-d and PTPR-s induces excitatory synapse formation.

Long Name

Netrin-G3 Ligand/Leucine-rich Repeat Containing 4B

Entrez Gene IDs

94030 (Human); 272381 (Mouse); 308571 (Rat)

Alternate Names

DKFZp761A179; HSM; Leucine Rich Repeat Containing 4B; Leucine-Rich Repeat-Containing Protein 4B; Leucine-Rich Repeats And Immunoglobulin-Like Domains 4; LRIG4; LRRC4B; Netrin-G3 Ligand; NGL3; NGL-3