Human NgR2/NgRH1 Antibody

Catalog # Availability Size / Price Qty
MAB2776
MAB2776-SP
Product Details
FAQs
Supplemental Products
Reviews (2)

Human NgR2/NgRH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NgR2/NgRH1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) NgR3 or rhNgR is observed.
Source
Monoclonal Mouse IgG2B Clone # 333413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NgR2/NgRH1
Ala28-Leu401
Accession # Q86UN3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NgR2/NgRH1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NgR2/NgRH1

NgR2, also known as reticulon 4 receptor-like 2 and NgRH1, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- as well as the C-terminus. Membrane-anchored NgR2 can be solubilized through the action of phospholipase or an unidentified MT-MMP to generate a 46 kDa soluble receptor. Mature human NgR2 shares 48% aa sequence identity with human NgR3. It also shares 96% aa sequence identity with mouse and rat mature NgR2.

Long Name
Nogo-66 Receptor-related Protein 2/Nogo-66 Receptor Homolog 1/Reticulon-4 Receptor-like 2
Entrez Gene IDs
349667 (Human); 269295 (Mouse)
Alternate Names
NgR2; NgRH1; reticulon 4 receptor-like 2; RTN4RL2

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Reviews for Human NgR2/NgRH1 Antibody

Average Rating: 3.5 (Based on 2 Reviews)

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Human NgR2/NgRH1 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human NgR2/NgRH1 Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human