Human Nicastrin Antibody

Catalog #: MAB53781
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB53781
MAB53781-SP

Detection of Human Nicastrin by Western Blot.

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All Nicastrin Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nicastrin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nicastrin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 716910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Nicastrin
Asn34-Glu669
Accession # Q92542

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Nicastrin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nicastrin Monoclonal Antibody (Catalog # MAB53781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Nicastrin at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nicastrin

Nicastrin (NCT) is a 150‑160 kDa member of the nicastrin family of proteins. It is a component of the aspartyl protease gamma -secretase complex and serves to stabilize and direct gamma -secretase components to proper positions in the plasma membrane. The gamma -secretase complex mediates the cleavage of intramembrane proteins such as notch-1 and APP. Mature human nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma -secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.

Entrez Gene IDs

23385 (Human); 59287 (Mouse); 289231 (Rat)

Alternate Names

anterior pharynx-defective 2; APH2; ATAG1874; KIAA0253APH2; NCSTN; Nicastrin

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Nicastrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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