Human NIK/MAP3K14 Antibody

Catalog #: MAB6888R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB6888R-100
MAB6888R-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NIK/MAP3K14 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NIK/MAP3K14 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 603917R

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NIK/MAP3K14
Glu769-Pro947
Accession # Q99558

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

immersion fixed paraffin-embedded sections of human pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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NIK/MAP3K14 in Human Pancreas. NIK/MAP3K14 was detected in immersion fixed paraffin-embedded sections of human pancrease using Mouse Anti-Human NIK/MAP3K14 Monoclonal Antibody (Catalog # MAB6888R) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine and endocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NIK/MAP3K14

NIK (NF kappa B inducing kinase), also called MAP3K14, is a widely expressed 947 amino acid (aa), ~100 kDa cytoplasmic protein of the MAP kinase family. NIK participates in a non-canonical NFkB signaling cascade in which its activity and expression are elevated by TNF and related ligands. NIK activates IKKa/b, which releases NFkB subunits for translocation to the nucleus. Within the region used as an immunogen, human NIK shares 94% and 95% aa sequence identity with mouse and rat NIK, respectively.

Long Name

NF-kappa-beta Inducing Kinase

Entrez Gene IDs

9020 (Human); 53859 (Mouse); 360640 (Rat)

Alternate Names

EC 2.7.11.25; FTDCR1B; HS; HSNIK; HSNIKserine/threonine protein-kinase; MAP3K14; mitogen-activated protein kinase kinase kinase 14; NIK; NIKNF-kappa-beta-inducing kinase; Serine/threonine-protein kinase NIK