Human NKCC1/SLC12A2 APC-conjugated Antibody

Catalog #: FAB8528A Datasheet / COA / SDS

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FAB8528A-025

Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry.

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Human NKCC1/SLC12A2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human NKCC1/SLC12A2 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 915911

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human NKCC1/SLC12A2
Met1-Ser1212
Accession # P55011

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 APC-conjugated Monoclonal Antibody (Catalog # FAB8528A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKCC1/SLC12A2

Solute Carrier, family 12 (potassium/chloride transporter), member 2 (SLC12A2) encodes NKCC1, which mediates sodium and chloride transport and reabsorption. It is a membrane protein that is important in maintaining proper ionic balance and cell volume. In contrast to the renal-specific sodium/potassium/chloride cotransporter SLC12A1, SLC12A2 is expressed in many tissues, including the basolateral membrane of secretory epithelia. It is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for SLC12A2.

Long Name

Sodium/Potassium/Chloride Transporter NKCC1

Entrez Gene IDs

6558 (Human); 20496 (Mouse); 83629 (Rat)

Alternate Names

Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; NKCC1; NKCC1MGC104233; SLC12A2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; solute carrier family 12 member 2