Home / NKG2A/CD159a / Human NKG2A/CD159a Antibody

Human NKG2A/CD159a Antibody

Catalog #: MAB1059
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1059-SP
MAB1059-100
MAB1059-500
Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry.
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Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2A/CD159a Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
Source
Monoclonal Mouse IgG2A Clone # 131411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2A and CD94
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKG2A/CD159a antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of NKG2A/CD159a antibody in HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP antibody by Flow Cytometry. View Larger

Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP by Flow Cytometry. HEK293 Human embryonic kidney cell line transfected with either (A) Human NKG2A/CD159a, or (B) Human NKG2B/CD159b and eGFP was stained with Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.

Long Name
Natural Killer G2A
Entrez Gene IDs
3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)
Alternate Names
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B

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Citations for Human NKG2A/CD159a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis
    Authors: Wang, J;Chai, Q;Lei, Z;Wang, Y;He, J;Ge, P;Lu, Z;Qiang, L;Zhao, D;Yu, S;Qiu, C;Zhong, Y;Li, BX;Zhang, L;Pang, Y;Gao, GF;Liu, CH;
    EMBO molecular medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTOF-reported
  2. Functional TRPA1 Channels Regulate CD56dimCD16+ NK Cell Cytotoxicity against Tumor Cells
    Authors: Scopelliti, F;Dimartino, V;Cattani, C;Cavani, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Extracellular Vesicle-Mediated Secretion of HLA-E by Trophoblasts Maintains Pregnancy by Regulating the Metabolism of Decidual NK Cells
    Authors: L Jiang, H Fei, X Jin, X Liu, C Yang, C Li, J Chen, A Yang, J Zhu, H Wang, X Fei, S Zhang
    International journal of biological sciences, 2021-10-22;17(15):4377-4395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain
    Authors: GT Hò, AA Celik, T Huyton, W Hiemisch, R Blasczyk, GS Simper, C Bade-Doedi
    Int J Mol Sci, 2020-06-19;21(12):.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Affinity Chromatography, ELISA Capture
  5. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8? T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia
    Authors: WC Pump, T Kraemer, T Huyton, GT Hò, R Blasczyk, C Bade-Doedi
    Int J Mol Sci, 2019-03-22;20(6):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Immunoprecipitation
  6. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age
    Authors: B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess
    Aging (Albany NY), 2019-01-27;11(2):724-740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Reduced CD160 expression contributes to impaired NK cell function and poor clinical outcomes in patients with HCC
    Authors: H Sun, J Xu, Q Huang, M Huang, K Li, K Qu, H Wen, R Lin, M Zheng, H Wei, W Xiao, R Sun, Z Tian, C Sun
    Cancer Res., 2018-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Negative regulation of tumor-infiltrating NK cell in clear cell renal cell carcinoma patients through the exosomal pathway
    Authors: Y Xia, Q Zhang, Q Zhen, Y Zhao, N Liu, T Li, Y Hao, Y Zhang, C Luo, X Wu
    Oncotarget, 2017-06-06;8(23):37783-37795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
    Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
    BMC Gastroenterol, 2015-12-10;15(0):173.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Molecular signatures and transcriptional regulatory networks of human immature decidual NK and mature peripheral NK cells.
    Authors: Wang F, Zhou Y, Fu B, Wu Y, Zhang R, Sun R, Tian Z, Wei H
    Eur J Immunol, 2014-06-11;44(9):2771-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.
    Authors: Serrano-Pertierra E, Cernuda-Morollon E, Brdicka T, Hooejsi V, Lopez-Larrea C
    J Leukoc Biol, 2014-05-06;96(3):437-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. MicroRNA editing facilitates immune elimination of HCMV infected cells.
    Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O
    PLoS Pathog, 2014-02-27;10(2):e1003963.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Irradiation-Induced Up-Regulation of HLA-E on Macrovascular Endothelial Cells Confers Protection against Killing by Activated Natural Killer Cells
    Authors: Isabelle Riederer, Wolfgang Sievert, Günther Eissner, Michael Molls, Gabriele Multhoff
    PLoS ONE
  14. NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease.
    Authors: Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L
    J. Leukoc. Biol., 2010-10-15;89(1):75-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008-08-01;181(3):2227-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
    Blood, 2007-04-01;109(7):2806-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
    Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
    J. Leukoc. Biol., 2005-06-03;78(2):359-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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