Human NKG2D/CD314 Antibody
Human NKG2D/CD314 Antibody Summary
Applications
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of NKG2D/CD314 in Human PBMCs gated on CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of Human NKG2D/CD314 by Flow Cytometry Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKG2D/CD314
NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.
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- Diefenbach, A. et al. (2001) Nature 413:165.
- NKG2D and its Ligands (2002) www.RnDSystems.com.
Product Datasheets
Citations for Human NKG2D/CD314 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Low‑dose valproic acid with low‑dose gemcitabine augments MHC class I‑related chain A/B expression without inducing the release of soluble MHC class I‑related chain A/B
Authors: Tomoharu Miyashita, Kenji Miki, Takashi Kamigaki, Isamu Makino, Hidehiro Tajima, Shinichi Nakanuma et al.
Oncology Letters
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Multiplex interrogation of the NK cell signalome reveals global downregulation of CD16 signaling during lentivirus infection through an IL-18/ADAM17-dependent mechanism
Authors: Sugawara S, Hueber B, Woolley G et al.
PLoS pathogens
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NKG2D ligand expression in Crohn's disease and NKG2D-dependent stimulation of CD8+ T cell migration
Authors: Kasper Vadstrup, Elisabeth Douglas Galsgaard, Helle Jensen, Lewis L. Lanier, James C. Ryan, Shih-Yu Chen et al.
Experimental and Molecular Pathology
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Inhibition of the B7-H3 immune checkpoint limits tumor growth by enhancing cytotoxic lymphocyte function
Authors: Young-hee Lee, Natalia Martin-Orozco, Peilin Zheng, Jing Li, Peng Zhang, Haidong Tan et al.
Cell Research
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CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7+ Tumor Cells
Authors: Xiaoxuan Zhuang, Eric O. Long
Cancer Immunology Research
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ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing
Authors: Maria Raffaella Zocchi, Caterina Camodeca, Elisa Nuti, Armando Rossello, Roberta Venè, Francesca Tosetti et al.
OncoImmunology
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NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human gamma δ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors
Authors: Guranda Chitadze, Marcus Lettau, Stefanie Luecke, Ting Wang, Ottmar Janssen, Daniel Fürst et al.
OncoImmunology
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CD16 pre-ligation by defucosylated tumor-targeting mAb sensitizes human NK cells to gamma c cytokine stimulation via PI3K/mTOR axis
Authors: Cristina Capuano, Chiara Pighi, Roberta Maggio, Simone Battella, Stefania Morrone, Gabriella Palmieri et al.
Cancer Immunology, Immunotherapy
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Downregulation of the stress-induced ligand ULBP1 following SV40 infection confers viral evasion from NK cell cytotoxicity
Authors: Yoav Bauman, Nir Drayman, Orly Ben-Nun-Shaul, Alon Vitenstein, Rachel Yamin, Yael Ophir et al.
Oncotarget
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Chronic stimulation desensitizes ?2-adrenergic receptor responses in natural killer cells
Authors: Jürgens, M;Claus, M;Wingert, S;Niemann, JA;Picard, LK;Hennes, E;Haasler, I;Hellwig, B;Overbeck, N;Reinders, J;Rahnenführer, J;Schedel, M;Capellino, S;Watzl, C;
European journal of immunology
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: Neutralization, ELISA Capture -
A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors
Authors: King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ;
Cancer immunology research
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata
Authors: R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar
Elife, 2023-03-17;12(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients
Authors: A Carmena Mo, Y Carpentier, F Lemaître, N Farzam-Kia, S Da Cal, JV Guimond, E Haddad, P Duquette, JM Girard, A Prat, C Larochelle, N Arbour
Multiple sclerosis and related disorders, 2023-01-26;71(0):104542.
Species: Human
Sample Types: Whole Cells
Applications: Blocking/Neutralizing -
Immunophenotype and antitumor activity of cytokine-induced killer cells from patients with hepatocellular carcinoma
Authors: CK Yang, CH Huang, CH Hu, JH Fang, TC Chen, YC Lin, CY Lin
PLoS ONE, 2023-01-04;18(1):e0280023.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
Cell Reports, 2022-09-27;40(13):111423.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation
Authors: MC Puzzolo, M Breccia, P Mariglia, G Colafigli, S Pepe, E Scalzulli, E Mariggiò, R Latagliata, A Guarini, R Foà
Journal of Clinical Medicine, 2022-09-23;11(19):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The metabolism of cells regulates their sensitivity to NK cells depending on p53 status
Authors: S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba
Scientific Reports, 2022-02-25;12(1):3234.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
Authors: N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba
Scientific Reports, 2022-01-25;12(1):1341.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway
Authors: HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang
International immunopharmacology, 2022-01-06;104(0):108497.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells
Authors: D Westhoff S, A Chakravort, M Hayes, W Hammerschm, B Sugden
MBio, 2021-11-16;0(0):e0224321.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
Authors: S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli
Cell Death & Disease, 2021-09-04;12(9):836.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells
Authors: X Zhong, H Liao, S Hu, K Luo, H Zhu
Molecular and cellular probes, 2021-07-12;59(0):101759.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing
Authors: E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim
PloS Pathogens, 2021-05-03;17(5):e1008807.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D
Authors: M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim
Viruses, 2020-07-20;12(7):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer
Authors: S Capellero, J Erriquez, C Melano, G Mesiano, S Genta, A Pisacane, G Mittica, E Ghisoni, M Olivero, MF Di Renzo, M Aglietta, D Sangiolo, G Valabrega
Sci Rep, 2020-04-15;10(1):6478.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NKG2D/DAP10 Signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity
Authors: KM Wilton, BL Overlee, DD Billadeau
J. Cell. Sci., 2019-07-18;0(0):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Cytotoxicity Assay, Stimulation -
Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival
Authors: A Gras Navar, H Espedal, JV Joseph, L Trachsel-M, M Bahador, B Tore Gjert, E Klæboe Kri, A Simonsen, H Miletic, P Øyvind Eng, MA Rahman, M Chekenya
Cancers (Basel), 2019-07-17;11(7):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
JCI Insight, 2019-07-11;4(13):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect)
Authors: E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun
Cancers (Basel), 2019-07-09;11(7):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
JCI Insight, 2019-06-11;5(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers
Authors: CF Amorim, NB Carvalho, JA Neto, SB Santos, MFR Grassi, LP Carvalho, EM Carvalho
J Immunol Res, 2019-02-28;2019(0):6574828.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age
Authors: B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess
Aging (Albany NY), 2019-01-27;11(2):724-740.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses
Authors: J Prévost, J Richard, H Medjahed, A Alexander, J Jones, JC Kappes, C Ochsenbaue, A Finzi
J. Virol., 2018-06-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells
Authors: YS Lee, W Heo, J Nam, YH Jeung, J Bae
J. Radiat. Res., 2018-05-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances ??T cell-mediated cytotoxicity via TCR and NKG2D
Authors: YM Dai, HY Liu, YF Liu, Y Zhang, W He
Immunology, 2018-03-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1
Authors: O Matalon, A Ben-Shmuel, J Kivelevitz, B Sabag, S Fried, N Joseph, E Noy, G Biber, M Barda-Saad
EMBO J., 2018-02-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors
Authors: S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme
Oncoimmunology, 2017-11-06;7(2):e1392426.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
Sci Rep, 2017-08-17;7(1):8661.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System
Authors: S Santaguida, A Richardson, DR Iyer, O M'Saad, L Zasadil, KA Knouse, YL Wong, N Rhind, A Desai, A Amon
Dev. Cell, 2017-06-19;41(6):638-651.e5.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells
Authors: X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh
Neoplasia, 2017-05-09;19(6):471-482.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells
Authors: Z Djaoud, LA Guethlein, A Horowitz, T Azzi, N Nemat-Gorg, D Olive, D Nadal, PJ Norman, C Münz, P Parham
J. Exp. Med., 2017-05-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Prognostic significance and functional implication of immune activating receptor NKG2D in gastric cancer
Authors: F Lin, C Dai, X Ge, W Tang, Y Lin, Y Wang, J Li
Biochem. Biophys. Res. Commun., 2017-04-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
EBI3 regulates the NK cell response to mouse cytomegalovirus infection
Authors: H Jensen, SY Chen, L Folkersen, GP Nolan, LL Lanier
Proc. Natl. Acad. Sci. U.S.A, 2017-01-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
Oncotarget, 2017-01-24;8(4):5954-5964.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
Oncotarget, 2017-01-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A functional polymorphism in the NKG2D gene modulates NK-cell cytotoxicity and is associated with susceptibility to Human Papilloma Virus-related cancers
Sci Rep, 2016-12-20;6(0):39231.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice
Authors: M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou
Oncotarget, 2016-08-02;7(31):49509-49526.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells.
Authors: Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E
AIDS Res Hum Retroviruses, 2016-07-20;33(2):93-100.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
PLoS ONE, 2015-05-07;10(5):e0125544.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
BMC Cancer, 2015-05-03;15(0):351.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Central nervous system acute lymphoblastic leukemia: role of natural killer cells.
Authors: Frishman-Levy L, Shemesh A, Bar-Sinai A, Ma C, Ni Z, Frenkel S, Muench V, Bruckmueller H, Vokuhl C, Debatin K, Eckert C, Stanulla M, Schrappe M, Campbell K, Loewenthal R, Schewe D, Hochman J, Meyer L, Kaufman D, Cario G, Porgador A, Izraeli S
Blood, 2015-04-20;125(22):3420-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A truncated human NKG2D splice isoform negatively regulates NKG2D-mediated function.
Authors: Karimi M, Aguilar O, Zou B, Bachmann M, Carlyle J, Baldwin C, Kambayashi T
J Immunol, 2014-08-04;193(6):2764-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dok1 and Dok2 proteins regulate natural killer cell development and function.
Authors: Celis-Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, Dalod M, Vivier E, Nunes J
EMBO J, 2014-06-24;33(17):1928-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells.
Authors: Molfetta R, Quatrini L, Capuano C, Gasparrini F, Zitti B, Zingoni A, Galandrini R, Santoni A, Paolini R
Eur J Immunol, 2014-06-17;44(9):2761-70.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.
Authors: Serrano-Pertierra E, Cernuda-Morollon E, Brdicka T, Hooejsi V, Lopez-Larrea C
J Leukoc Biol, 2014-05-06;96(3):437-45.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Natural killer cells induce eosinophil activation and apoptosis.
Authors: Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, Duez C
PLoS ONE, 2014-04-11;9(4):e94492.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas.
Authors: Sangiolo, Dario, Mesiano, Giulia, Gammaitoni, Loretta, Leuci, Valeria, Todorovic, Maja, Giraudo, Lidia, Cammarata, Cristina, Dell'Aglio, Carmine, D'Ambrosio, Lorenzo, Pisacane, Alberto, Sarotto, Ivana, Miano, Sara, Ferrero, Ivana, Carnevale-Schianca, Fabrizio, Pignochino, Ymera, Sassi, Francesc, Bertotti, Andrea, Piacibello, Wanda, Fagioli, Franca, Aglietta, Massimo, Grignani, Giovanni
Cancer Res, 2013-12-19;74(1):119-29.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions.
Authors: Windheim, Mark, Southcombe, Jennifer, Kremmer, Elisabet, Chaplin, Lucy, Urlaub, Doris, Falk, Christin, Claus, Maren, Mihm, Janine, Braithwaite, Myles, Dennehy, Kevin, Renz, Harald, Sester, Martina, Watzl, Carsten, Burgert, Hans-Ger
Proc Natl Acad Sci U S A, 2013-11-11;110(50):E4884-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
J Exp Med, 2013-11-04;210(12):2675-92.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The road to memory: an early rest for the long journey.
Authors: Ho, Liam Poc, Yit, Pui San, Ng, Lee Hui, Linn, Yeh Chin, Zhao, Yi, Sun, Li, Ling, Khoon Li, Chai Koh, Mickey B, Monica Shih, Meng-Chu, Li, Shang, Wang, Xue Ying, Tien, Sim Leng, Goh, Yeow Tee
J Immunol, 2013-11-01;191(11):5603-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling.
Authors: Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R
Eur J Immunol, 2013-06-18;43(9):2473-83.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
Authors: Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, Choi J, Choi I, Yun C, Kim J, Yee C, Kumar V, Lee K
Cancer Res, 2013-04-11;73(8):2598-607.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Mechanisms of the antitumor activity of human Vgamma9Vdelta2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma.
Authors: Di Carlo E, Bocca P, Emionite L, Cilli M, Cipollone G, Morandi F, Raffaghello L, Pistoia V, Prigione I
Mol Ther, 2013-03-12;21(5):1034-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dedicator of cytokinesis 8 interacts with talin and Wiskott-Aldrich syndrome protein to regulate NK cell cytotoxicity.
Authors: Ham, Hyoungju, Guerrier, Sabrice, Kim, JungJin, Schoon, Renee A, Anderson, Erik L, Hamann, Michael, Lou, Zhenkun, Billadeau, Daniel D
J Immunol, 2013-03-01;190(7):3661-9.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Analysis of Vdelta1 T cells in clinical grade melanoma-infiltrating lymphocytes.
Authors: Donia M, Ellebaek E, Andersen M, Straten P, Svane I
Oncoimmunology, 2012-11-01;1(8):1297-1304.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins.
Authors: Brown A, Dobbie I, Alakoskela J, Davis I, Davis D
Blood, 2012-09-10;120(18):3729-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
Cancer Res, 2012-08-21;72(21):5463-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation.
Eur J Immunol, 2012-08-01;42(8):2142-51.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
Authors: Morandi F, Prigione I, Airoldi I
Eur. J. Immunol., 2012-06-01;42(6):1547-52.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Lab. Invest., 2012-03-26;92(7):967-77.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
PLoS ONE, 2012-02-22;7(2):e31959.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation.
Authors: Andresen L, Skovbakke SL, Persson G, Hagemann-Jensen M, Hansen KA, Jensen H, Skov S
J. Immunol., 2012-01-06;188(4):1847-55.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D.
Authors: Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, Schadendorf D, Paschen A
Cancer Res., 2011-11-18;72(2):460-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
Authors: Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A
Blood, 2011-03-15;117(18):4778-86.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization -
Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
Authors: Inoue T, Tashiro Y, Takeuchi M
Int. J. Oncol., 2011-03-03;38(5):1299-306.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling.
Authors: Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R
Diabetes, 2011-01-26;60(3):857-66.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease.
Authors: Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L
J. Leukoc. Biol., 2010-10-15;89(1):75-84.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
Immunol. Lett., 2010-10-07;134(2):167-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytotoxicity of activated natural killer cells against pediatric solid tumors.
Authors: Cho D, Shook DR, Shimasaki N
Clin. Cancer Res., 2010-06-11;16(15):3901-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement.
Authors: Maniar A, Zhang X, Lin W, Gastman BR, Pauza CD, Strome SE, Chapoval AI
Blood, 2010-06-02;116(10):1726-33.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
SHP-2 expression negatively regulates NK cell function.
Authors: Purdy AK, Campbell KS
J. Immunol., 2009-11-13;183(11):7234-43.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
Authors: Markel G, Seidman R, Besser MJ
PLoS ONE, 2009-05-19;4(5):e5597.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
Authors: Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
Int. J. Cancer, 2009-04-01;124(7):1594-604.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
Blood, 2009-01-05;113(13):2955-64.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
J. Mol. Biol., 2008-09-16;384(5):1143-56.
Species: Human
Sample Types: Peptide, Whole Cells
Applications: Neutralization, Surface Plasmon Resonance -
An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
J. Immunol., 2008-08-01;181(3):2227-37.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Functional Assay, IHC-Fr -
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Mol. Immunol., 2008-07-26;45(15):3917-25.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
Biochem. Biophys. Res. Commun., 2008-04-04;370(4):578-83.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells.
Authors: Kim YJ, Han MK, Broxmeyer HE
Blood, 2007-11-16;111(3):1378-86.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
Blood, 2007-10-18;111(3):1309-17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy.
Authors: Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH, Mackall CL
J. Immunol., 2007-10-01;179(7):4910-8.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
Authors: Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT
Proc. Natl. Acad. Sci. U.S.A., 2006-07-18;103(30):11258-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC, Neutralization -
Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
Blood, 2006-01-05;107(9):3617-23.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
Cancer Res., 2005-07-15;65(14):6409-17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
J. Leukoc. Biol., 2005-06-03;78(2):359-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas
Authors: Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio et al.
Blood
-
Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium
Authors: Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara et al.
eLife
-
The septin cytoskeleton regulates natural killer cell lytic granule release
Authors: Prasad V. Phatarpekar, Brittany L. Overlee, Alexander Leehan, Katelynn M. Wilton, Hyoungjun Ham, Daniel D. Billadeau
Journal of Cell Biology
-
A Trypanosoma brucei beta 3 glycosyltransferase superfamily gene encodes a beta 1-6 GlcNAc-transferase mediating N-glycan and GPI anchor modification
Authors: Samuel M. Duncan, Rupa Nagar, Manuela Damerow, Dmitry V. Yashunsky, Benedetta Buzzi, Andrei V. Nikolaev et al.
Journal of Biological Chemistry
-
Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells
Authors: S Sasawatari, Y Okamoto, A Kumanogoh, T Toyofuku
J. Immunol., 2020-01-22;0(0):.
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Sensitizing primary acute lymphoblastic leukemia to natural killer cell recognition by induction of NKG2D ligands
Authors: Laura Jardine, Sophie Hambleton, Venetia Bigley, Sarah Pagan, Xiao-Nong Wang, Matthew Collin
Leukemia & Lymphoma
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