Human NKG2D/CD314 APC-conjugated Antibody

Catalog #: FAB1392A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1392A-100

Detection of NKG2D/CD314 in NK cells by Flow Cytometry.

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All NKG2D/CD314 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2D/CD314 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NKG2D/CD314 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1046002

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NKG2D/CD314
Phe78-Val216
Accession # P26718

Formulation

Supplied in a saline solution containing Tris and Sodium Azide

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

Day 15 expanded and activated NK cells, PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKG2D/CD314 in NK cells by Flow Cytometry. Day 15 expanded and activated NKcells were stained with (A) MouseAnti-Human NKG2D/CD314APC-conjugated MonoclonalAntibody (Catalog # FAB1392A)vs (B) Mouse IgG2B APC-conjugated antibody(IC0041A) along withRabbit Anti-Human NCAM-1/CD56 PE-conjugatedMonoclonal Antibody (FAB24086P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of NKG2D/CD314 in PBMC cells by Flow Cytometry. PBMCs were stained with (A) MouseAnti-Human NKG2D/CD314APC-conjugated MonoclonalAntibody (Catalog # FAB1392A)vs (B) Mouse IgG2B APC-conjugated antibody(IC0041A) along withRabbit Anti-Human NCAM-1/CD56 PE-conjugatedMonoclonal Antibody (FAB24086P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

References

Li, P. et al. (2001) Nature Immunol. 2:443.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Cosman, D. et al. (2001) Immunity 14:123.
Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
Wu, J. et al. (1999) Science 285:730.
Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
Groh, V. et al. (2001) Nature Immunol. 2:255.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
Diefenbach, A. et al. (2001) Nature 413:165.
NKG2D and its Ligands (2002) www.rndsystems.com.

Long Name

Natural Killer G2D

Entrez Gene IDs

22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)

Alternate Names

CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence