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Human NKG2D/CD314 APC-conjugated Antibody

Catalog #: FAB139A
11 Citations Datasheet / COA / SDS
Clone 149810 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB139A-025
FAB139A-100
Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2D/CD314 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 149810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKG2D/CD314 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 APC-conjugated Monoclonal Antibody (Catalog # FAB139A) or (B) Mouse IgG1APC Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human NKG2D/CD314 by Flow Cytometry View Larger

Detection of Human NKG2D/CD314 by Flow Cytometry TL1A induces the growth of PLZF+ cells.(A) PBLs were purified from healthy donors, CFSE stained and stimulated for 6 days with combinations of IL-12 (4 ng/mL), IL-15 (10 ng/mL), IL-18 (40 ng/mL), TL1A (100 ng/mL) and TL1AAb (1 µg/mL). Upper panel shows gating on live cells and (%) proliferating cells as seen by less CFSE/cell. Lower panels show staining of different surface molecules. Note that for CD8, both CD8+ (bright+dim) and CD8dim cells are shown (intermediate (%) in grey); see text for elaboration. Data are representative of three different experiments with cells from three separate donors. (B) PBLs were extracted after 6 days of stimulation with the cytokines listed, and PLZF was detected in whole cell extracts or cytoplasmic/nuclear extracts. Data are essentially representative of three different experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085793), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

References
  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

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Citations for Human NKG2D/CD314 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Fumarate Upregulates Surface Expression of ULBP2/ULBP5 by Scavenging Glutathione Antioxidant Capacity
    Authors: RI Høgh, A Droujinine, SH Møller, SD Jepsen, M Mellergaar, L Andresen, S Skov
    J. Immunol., 2020-03-06;0(0):.
  2. Traditional Thai Massage Promoted Immunity in the Elderly via Attenuation of Senescent CD4+ T Cell Subsets: A Randomized Crossover Study
    Authors: K Sornkayasi, A Jumnainson, W Phoksawat, W Eungpinich, C Leelayuwat
    International Journal of Environmental Research and Public Health, 2021-03-19;18(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  3. Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells
    Authors: Sofie H. Møller, Maiken Mellergaard, Mikkel Madsen, Amaia V. Bermejo, Stine D. Jepsen, Marie H. Hansen et al.
    Frontiers in Immunology
  4. In the Absence of a TCR Signal IL-2/IL-12/18-Stimulated gammadelta T Cells Demonstrate Potent Anti-Tumoral Function Through Direct Killing and Senescence Induction in Cancer Cells
    Authors: K Schilbach, C Welker, N Krickeberg, C Kai beta er, S Schleicher, H Hashimoto
    Cancers (Basel), 2020-01-04;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Transforming growth factor-beta 1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands
    Authors: HYUNKEUN SONG, YEONYE KIM, GABIN PARK, YEONG-SEOK KIM, SEONGHAN KIM, HYUN-KYUNG LEE et al.
    International Journal of Molecular Medicine
  6. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
    Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
    J Immunol, 2014-07-14;193(4):1654-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. TL1A induces TCR independent IL-6 and TNF-alpha production and growth of PLZF(+) leukocytes.
    Authors: Reichwald K, Jorgensen T, Tougaard P, Skov S
    PLoS ONE, 2014-01-08;9(1):e85793.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
    Authors: Markel G, Seidman R, Besser MJ
    PLoS ONE, 2009-05-19;4(5):e5597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CD27 defines phenotypically and functionally different human NK cell subsets.
    Authors: Vossen MT, Matmati M, Hertoghs KM, Baars PA, Gent MR, Leclercq G, Hamann J, Kuijpers TW, van Lier RA
    J. Immunol., 2008-03-15;180(6):3739-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells.
    Authors: Kim YJ, Han MK, Broxmeyer HE
    Blood, 2007-11-16;111(3):1378-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Phosphostim-activated gamma delta T cells kill autologous metastatic renal cell carcinoma.
    Authors: Viey E, Fromont G, Escudier B, Morel Y, Da Rocha S, Chouaib S, Caignard A
    J. Immunol., 2005-02-01;174(3):1338-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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