Human NKp46/NCR1 Antibody

Catalog #: MAB1850R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1850R-100
MAB1850R-SP

Detection of NKp46/NCR1 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKp46/NCR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NKp46/NCR1 in ELISA.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 195314R

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse T cell hybridoma transfected with human NKp46/NCR1
Accession # O76036

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC

Agonist Activity

Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. The ED50 for this effect is 30.0 -300 ng/mL

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKp46/NCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850R) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Agonist Activity

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Induce IFN-gamma secretion by Human NKp46/NCR1 antibody Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. The ED50 for this effect is 30.0 -300 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

References

Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Arnon, T. et al. (2004) Blood 103:664.
Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
Mandelboim, O. et al. (2001) Nature 409:1055.
Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.

Entrez Gene IDs

9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)

Alternate Names

CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)