Human NLRP3/NALP3 Antibody

Catalog #: MAB67891 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB67891-100
MAB67891-SP

Detection of Human NLRP3/NALP3 by Western Blot.

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All NLRP3/NALP3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NLRP3/NALP3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NALP-3/NALP3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 447609

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NALP-3/NALP3
Met3-Val223
Accession # Q96P20

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NLRP3/NALP3 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 3 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB67891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NLRP3/NALP3 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP3/NALP3

NLRP3 (NAcht Leucine-rich repeat Protein 3), also known as NALP3, CIAS1, PYPAF or Cryopyrin is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Human NLRP3 contains an N-terminal Pyrin domain (aa 1-93) followed by a Nacht region (aa 220-536), and seven LRRs (aa 740-991). Within the sequence used as an immunogen, human NLRP3 shares 76% and 75% aa identity with mouse and rat NLRP3, respectively. Isoforms of 979, 979, 922 and 719 aa lack aa 720–776, aa 836-892, both regions, or terminate at aa 719.

Long Name

NLR Family, Pyrin Domain Containing 2

Entrez Gene IDs

114548 (Human); 216799 (Mouse); 287362 (Rat)

Alternate Names

AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1