Human Nogo-A aa 566-748 Antibody Summary
Val566-Phe748
Accession # Q9NQC3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Nogo‑A in Rat Cortical Stem Cells. Nogo-A was detected in immersion fixed 7 day differentiated rat cortical stem cells using 10 µg/mL Sheep Anti-Human Nogo-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3515) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-A
Human Nogo-A (also reticulon-4) is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1-4). In human, Nogo exists in five isoforms (5-7). The full length human form (Nogo-A) is 1192 aa in length and contains a 1018 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa connecting loop. This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (8-10). Two other areas in the N-terminus have also been discovered to have bioactivity (8, 11, 12). Based on rat, aa 57-184 in human (aa 59-172 in rat) should block fibroblast spreading, while aa 566-748 in human (aa 544-725 in rat) block neurite outgrowth and block fibroblast spreading (8, 12, 13). The exact topology of Nogo-A is unclear. The N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (13-15). Alternatively, the loop region and N-terminus may be on the same side of the membrane (3, 8). The four additional isoforms are shorter than Nogo-A (199 aa [Nogo-C], 373 aa [Nogo-B], 392 aa and 986 aa, respectively) (7). Although highly divergent, all contain the same C-terminal stretch, aa 1005-1192. Both Nogo-B and C are reported to complex with Nogo-A (16). Notably, Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (12, 16-18). Human Nogo-A is 78% aa identical to mouse and rat Nogo-A overall, with 98% aa identity in the loop region and approximately 80% aa identity in the aa 566-748 segment.
- Oertle, T. et al. (2003) FASEB J. 17:1238.
- GrandPre, T. et al. (2000) Nature 403:439.
- Yan, R. et al. (2006) Cell. Mol. Life Sci. 63:877.
- Teng, F.Y.H. et al. (2004) J. Neurochem. 89:801.
- Chen, M.S. et al. (2000) Nature 403:434.
- Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
- GenBank Accession # Q9NQC3.
- Oertle, T. et al. (2003) J. Neurosci. 23:5393.
- Fournier, A.E. et al. (2001) Nature 409:341.
- Wang, K.C. et al. (2002) Nature 420:74.
- Prinjha, R. et al. (2000) Nature 403:384.
- Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
- Li, M. et al. (2004) Eur. J. Biochem. 271:3512.
- Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
- Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
- Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
- Wang, X. et al. (2002) J. Neurosci. 22:5505.
- Acevedo, L. et al. (2004) Nat. Med. 10:382.
Product Datasheets
Citations for Human Nogo-A aa 566-748 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system
Authors: Jae Young Lee, Min Joung Kim, Lijun Li, Alexander A. Velumian, Pei Mun Aui, Michael G. Fehlings et al.
Scientific Reports
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A proteolytic C-terminal fragment of Nogo-A (reticulon-4A) is released in exosomes and potently inhibits axon regeneration
Authors: Y Sekine, JA Lindborg, SM Strittmatt
Nat. Med., 2019-11-20;295(8):2175-2183.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination
Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang
PLoS ONE, 2017-12-18;12(12):e0189380.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Protein microarray analysis identifies cyclic nucleotide phosphodiesterase as an interactor of Nogo-A.
Authors: Sumiyoshi K, Obayashi S, Tabunoki H
Neuropathology, 2009-06-07;30(1):7-14.
Species: Mouse, Rat
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot
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