Human Nogo Receptor/NgR Antibody Summary
Cys27-Ser447
Accession # Q9BZR6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo Receptor/NgR
Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid residues (aa) precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).
- Fournier, A.E. et al. (2001) Nature 409:341.
- GrandPre, T. et al. (2002) Nature 417:547.
- Wang, K.C. et al. (2002) Nature 420:74.
- Barton, W.A. et al. (2003) EMBO Journal 22:3291.
- Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
- Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.
Product Datasheets
Citations for Human Nogo Receptor/NgR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Environmental cues from neural crest derivatives act as metastatic triggers in an embryonic neuroblastoma model
Authors: D Ben Amar, K Thoinet, B Villalard, O Imbaud, C Costechare, L Jarrosson, F Reynaud, J Novion Duc, Y Couté, JF Brunet, V Combaret, N Corradini, C Delloye-Bo, V Castellani
Nature Communications, 2022-05-10;13(1):2549.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
PlexinA2 and CRMP2 Signaling Complex is Activated by Nogo-A-Liganded NgR1 to Restrict Corticospinal Axon Sprouting after Trauma
Authors: Y Sekine, PT Algarate, WBJ Cafferty, SM Strittmatt
J. Neurosci., 2019-02-25;0(0):.
Applications: ICC -
LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand.
Authors: Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K
Mol Cell Neurosci, 2014-07-15;61(0):211-8.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: ICC -
The Nogo Receptor NgR1 Mediates Infection by Mammalian Reovirus
Authors: Jennifer L. Konopka-Anstadt, Bernardo A. Mainou, Danica M. Sutherland, Yuichi Sekine, Stephen M. Strittmatter, Terence S. Dermody
Cell Host & Microbe
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Increased hippocampal NgR1 signaling machinery in aged rats with deficits of spatial cognition
Authors: Heather D. VanGuilder Starkey, William E. Sonntag, Willard M. Freeman
European Journal of Neuroscience
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Nogo-66 inhibits adhesion and migration of microglia via GTPase Rho pathway in vitro.
Authors: Yan J, Zhou X, Guo JJ, Mao L, Wang YJ, Sun J, Sun LX, Zhang LY, Zhou XF, Liao H
J. Neurochem., 2012-01-23;120(5):721-31.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ELISA Development, ICC, Western Blot -
The carboxyl-terminal region of Crtac1B/LOTUS acts as a functional domain in endogenous antagonism to Nogo receptor-1.
Authors: Kurihara Y, Arie Y, Iketani M, Ito H, Nishiyama K, Sato Y, Nakamura F, Mizuki N, Goshima Y, Takei K
Biochem. Biophys. Res. Commun., 2012-01-18;418(2):390-5.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists.
Authors: Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P
J. Biol. Chem., 2008-04-14;283(24):16641-52.
Species: Human
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Immunoprecipitation, Western Blot
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