Human Notch-2 Intracellular Domain Antibody

Name: Human Notch-2 Intracellular Domain Antibody
Brand: R&D Systems
SKU: MAB3735
Rating: 3.7 (3 reviews)

Catalog #: MAB3735 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3735
MAB3735-SP

All Notch-2 Antibodies

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Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Notch-2 Intracellular Domain Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch-2 intracellular domain (ICD) in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Notch-1 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, rhNotch-3 ECD, or rhNotch‑4 ICD.

Source

Monoclonal Rat IgG_2A Clone # 411801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Notch‑2
Asp2063-Pro2413
Accession # Q04721.3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Notch‑2 Intracellular Domain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-2

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180 kDa disulfide-linked extracellular domain (ECD) (likely aa 25-1538) and a 110 kDa membrane bound truncated segment (likely aa 1539-2471). Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa 1697-2471), a potential nuclear transcription factor. In the nucleus, NICD regulates CSL pathways. When NICD is phosphorylated, it is deactivated. Over aa 2063-2413, human Notch-2 is 94% and 89% aa identical to dog and mouse Notch-2, respectively.

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Alternate Names

AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2