Human Notch-4 Intracellular Domain Antibody Summary
rrNotch‑2 EC, or rrDelta-1 is observed.
Gly1778-Lys2003
Accession # Q99466
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Notch‑4 in Human HUVEC. Notch-4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Notch-4 Intracellular Domain Monoclonal Antibody (Catalog # MAB3847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-4
Notch-4 is a 250 kDa member of the NOTCH family of proteins. It is primarily expressed on endothelium. Human Notch-4 is a 1980 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1424 aa extracellular domain (ECD) and a 535 aa cytoplasmic region. Notch-4 undergoes proteolytic processing at multiple sites. There is probable cleavage of the ECD by furin in the Golgi between aa 1310‑1410. This creates a mature, potentially disulfide-linked heterodimer at the cell surface. Upon ligand binding, two sequential cleavages occur, one in the ECD after Ala1431, and a second in the TM domain after Lys1466. This creates a soluble cytoplasmic fragment that interacts with RBP-J kappa and activates genes associated with HES-1. Over cytoplasmic aa 1778‑2003, human Notch‑4 is 78% aa identical to mouse Notch-4.
Product Datasheets
Citation for Human Notch-4 Intracellular Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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KSHV-induced notch components render endothelial and mural cell characteristics and cell survival.
Authors: Liu R, Li X, Tulpule A, Zhou Y, Scehnet JS, Zhang S, Lee JS, Chaudhary PM, Jung J, Gill PS
Blood, 2009-11-24;115(4):887-95.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
