Home / Notch-4 / Human Notch-4 Intracellular Domain Antibody

Human Notch-4 Intracellular Domain Antibody

Catalog #: AF3847
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3847
AF3847-SP
Notch‑4 in SH‑SY5Y Human Cell Line.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Notch-4 Intracellular Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human Notch-4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Notch-1 Intracellular Domain and rhNotch-2 Intracellular Domain is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Notch-4 Intracellular Domain
Gly1778-Lys2003
Accession # Q99466
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Notch‑4 Intracellular Domain
Immunocytochemistry
5-15 µg/mL
Immersion fixed SH‑SY5Y human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Notch‑4 in SH‑SY5Y Human Cell Line. Notch‑4 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Notch‑4 Intracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3847) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-4

Notch-4 is a 250 kDa heterodimeric membrane protein with a C-terminal transmembrane fragment covalently linked to an N-terminal extracellular fragment. It functions as a receptor for transmembrane ligands such as the Jagged and Delta proteins. Upon ligand binding, it undergoes sequential proteolytic processing to release the intracellular domain (NICD), which is translocated to the nucleus where it activates target genes. In the region used as immunogen, human and mouse Notch-4 share 78% amino acid sequence homology.

Entrez Gene IDs
4855 (Human); 18132 (Mouse); 406162 (Rat)
Alternate Names
INT3; neurogenic locus notch homolog protein 4; notch 4; Notch homolog 4; NOTCH3; Notch4; Notch-4

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Notch-4 Intracellular Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
    Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
    Br. J. Dermatol., 2011-05-17;164(6):1228-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Delineation of a cellular hierarchy in lung cancer reveals an oncofetal antigen expressed on tumor-initiating cells.
    Authors: Damelin M, Geles KG, Follettie MT, Yuan P, Baxter M, Golas J, Dijoseph JF, Karnoub M, Huang S, Diesl V, Behrens C, Choe SE, Rios C, Gruzas J, Sridharan L, Dougher M, Kunz A, Hamann PR, Evans D, Armellino D, Khandke K, Marquette K, Tchistiakova L, Boghaert ER, Abraham RT, Wistuba II, Zhou BB
    Cancer Res., 2011-05-03;71(12):4236-46.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Notch-4 Intracellular Domain Antibody

There are currently no reviews for this product. Be the first to review Human Notch-4 Intracellular Domain Antibody and earn rewards!

Have you used Human Notch-4 Intracellular Domain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review