Human NPRC/NPR3 Antibody

Catalog #: MAB102331 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB102331-100
MAB102331-SP

Detection of NPRC/NPR3 in HEK293 cells by Flow Cytometry.

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All NPRC/NPR3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NPRC/NPR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NPRC/NPR3 in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1060830

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human NPRC/NPR3
Met1-Glu481
Accession # P17342

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

NCI‑H460 human large cell lung carcinoma cell line, OVCAR‑3 human ovarian carcinoma cell line

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human NPR3 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NPRC/NPR3 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human NPR3 and eGFP were stained with either (A) Mouse Anti-Human NPRC/NPR3 Monoclonal Antibody (Catalog # MAB102331) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Western Blot

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Detection of Human NPRC/NPR3 by Western Blot. Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line, OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NPRC/NPR3 Monoclonal Antibody (Catalog # MAB102331) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NPRC/NPR3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRC/NPR3

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPRC or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mature human NPR3 is a type I transmembrane glycoprotein that contains a 455 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 37 aa cytoplasmic region. Within the ECD, human NPR3 shares 92% aa sequence identity with mouse and rat NPR3. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).

References

Potter, L.R. et al. (2006) Endocr. Rev. 27:47.
Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081.
Thomas, G. et al. (2003) J. Biol. Chem. 278:50563.
Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.

Long Name

Natriuretic Peptide Receptor C

Entrez Gene IDs

4883 (Human); 18162 (Mouse); 25339 (Rat)

Alternate Names

ANP-C; ANPR-C; ANPRCFLJ14054; Atrial natriuretic peptide clearance receptor; atrial natriuretic peptide receptor 3; Atrial natriuretic peptide receptor type C; C5orf23; chromosome 5 open reading frame 23; GUCY2B; lgj; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C); NPR3; NPRC; NPR-C; stri