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Human OCIL/CLEC2d Antibody

Catalog #: AF3480
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3480
AF3480-SP
Detection of OCIL/CLEC2d in Raji Human Cell Line by Flow Cytometry.
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Citations (2)
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Human OCIL/CLEC2d Antibody Summary

Species Reactivity
Human
Specificity
Detects human OCIL/CLEC2d in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with recombinant human (rh) CLEC2B, and rhCLEC2A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human OCIL/CLEC2d
Ser57-Val191
Accession # Q9UHP7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human OCIL/CLEC2d
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of OCIL/CLEC2d antibody in Raji Human Cell Line antibody by Flow Cytometry. View Larger

Detection of OCIL/CLEC2d in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human OCIL/CLEC2d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3480, filled histo­gram) or control antibody (Catalog # AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCIL/CLEC2d

Human OCIL/CLEC2d, also known as Lectin-Like Transcript-1 (LLT-1), is a type II transmembrane protein belonging to the natural killer (NK) cell receptor group of the C-type lectin superfamily. It is found on hematopoietic cells, osteoblasts, and chondrocytes. By alternative splicing, at least three isoforms exist. Isoform 1 is synthesized as a 191 amino acid (aa) precursor with a C-terminal 132 aa extracellular domain (ECD) that contains the C-type lectin domain. The aa sequence of human OCIL isoform 1 ECD is 49% and 50% identical to the mouse and rat OCIL ECD, respectively. Human OCIL preferentially binds high molecular weight sulfated glycosaminoglycans and is a ligand for the single human NKR-P1A receptor (CD161). Human OCIL blocks osteoclast differentiation. Engagement of OCIL with CD161 on NK cells also inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion.

Long Name
Osteoclast Inhibitory Lectin
Entrez Gene IDs
29121 (Human); 93694 (Mouse)
Alternate Names
CLAXLLT-1; CLEC2D; C-type lectin domain family 2 member D; C-type lectin domain family 2, member D; C-type lectin related f; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT1; LLT1Osteoclast inhibitory lectin; OCIL; OCILmember D

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Citations for Human OCIL/CLEC2d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Expression of lectin-like transcript-1 in human tissues
    Authors: A Llibre, L Garner, A Partridge, GJ Freeman, P Klenerman, CB Willberg
    F1000Res, 2016-12-29;5(0):2929.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation
    Authors: Alba Llibre, Constantino López-Macías, Teresa Marafioti, Hema Mehta, Amy Partridge, Carina Kanzig et al.
    The Journal of Immunology

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