Human Olig1, 2, 3 PE‑conjugated Antibody

Catalog #: IC2230P Datasheet / COA / SDS

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IC2230P

Detection of Olig1, 2, 3 in A172 Human Cell Line by Flow Cytometry.

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All Olig 1, 2, 3 Antibodies

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Human Olig1, 2, 3 PE‑conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Olig1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) Olig2 and 50-100% cross-reactivity with rhOlig3.

Source

Monoclonal Mouse IgG_2A Clone # 257224

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Olig1
aa 1-272

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Olig1, 2, 3 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of Olig1, 2, 3 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human Olig1, 2, 3 PE-conjugated Monoclonal Antibody (Catalog # IC2230P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Olig 1, 2, 3

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adults, expression of Olig3 is primarily detected in neural tissues.

Long Name

Oligodendrocyte Lineage Gene

Alternate Names

bHLHb6; bHLHe21; Olig 1, 2, 3