Human Olig1 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Olig1 in Human Glioma. Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Olig1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Olig1
Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.
Product Datasheets
Citations for Human Olig1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Hedgehog Signalling Modulates Immune Response and Protects against Experimental Autoimmune Encephalomyelitis
Authors: A Ballester, A Guijarro, B Bravo, J Hernández, R Murillas, MI Gallego, S Ballester
International Journal of Molecular Sciences, 2022-03-15;23(6):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Highly efficient manipulation of nervous system gene expression with NEPTUNE
Authors: Katrin Mangold, Jan Mašek, Jingyan He, Urban Lendahl, Elaine Fuchs, Emma R. Andersson
Cell Reports Methods
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Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity
Authors: S Yang, S Li, XJ Li
Cell Rep, 2018-12-04;25(10):2653-2659.e3.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Lung tumors with distinct p53 mutations respond similarly to p53 targeted therapy but exhibit genotype-specific statin sensitivity
Authors: Frances K. Turrell, Emma M. Kerr, Meiling Gao, Hannah Thorpe, Gary J. Doherty, Jake Cridge et al.
Genes & Development
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Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.
Authors: Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C
J Neurosci, 2015-01-07;35(1):4-20.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Reduced Morg1 expression in ischemic human brain.
Authors: Haase D, Keiner S, Mawrin C, Wolf G
Neurosci. Lett., 2009-03-18;455(1):46-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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Section co-stained with anti-Olig1 (in green), anti-Olig2 (in magenta) and anti-Pdgfra(not shown).
I was expecting some degree of co-localisation in Olig1 and Olig2. Olig1 seems to be non specific and not to have worked.
