Human OSMR beta Antibody

Catalog #: MAB4389 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4389
MAB4389-SP

Detection of OSM R beta in HeLa Human Cell Line by Flow Cytometry.

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Human OSMR beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human OSM R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse OSM R beta is observed.

Source

Monoclonal Mouse IgG₁ Clone # 469221

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OSM R beta
Glu28-Ser739
Accession # Q99650

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human OSM R beta (Catalog # 4389-OR) under non-reducing conditions only

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB43891).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Knockout Validated

OSM R beta is specifically detected in HeLa human parental cell line but is not detectable in OSM R beta knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of OSM R beta in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB4389, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Knockout Validated

OSM R beta Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

View Larger

OSM R beta Specificity is Shown by Flow Cytometry in Knockout Cell Line. OSM R beta knockout HeLa human cervical carcinoma cell line was stained with Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB4389, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the OSM R beta knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Knockout Validated

Western Blot Shows Human OSM R beta Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human OSM R beta Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and OSM R beta knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB4389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for OSM R beta at approximately 45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OSMR beta

OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).

References

Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379.
Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39.
Gearing, D.P. et al. (1992) Science 255:1434.
Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
Tanaka, M. et al. (2003) Blood 102:3154.
Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.

Long Name

Oncostatin M Receptor beta

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Alternate Names

IL-31 receptor subunit beta; IL-31R subunit beta; IL-31RB; IL-31R-beta; Interleukin-31 receptor subunit beta; MGC150627; MGC75127; oncostatin M receptor; oncostatin-M specific receptor beta subunit; oncostatin-M-specific receptor subunit beta; OSM R beta; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1