Human Osteocalcin Antibody

Catalog #: MAB113271 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB113271-100
MAB113271-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Osteocalcin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Osteocalcin in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 190124

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Osteocalcin/KLH containing peptide
Accession # P02818

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB11327).This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Osteocalcin DuoSet ELISA Kit (Catalog # DY1419-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Osteocalcin ELISA Standard Curve. Recombinant Human Osteocalcin protein was serially diluted 2-fold and captured by Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB11327) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB113271) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteocalcin

Osteocalcin, also known as Bone gamma -Carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage (1). It has been frequently used as a marker for osteoblast lineage cells.

References

Lian, J.B. et al. (1999) Vitamin. Horm. 55:443.

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Entrez Gene IDs

632 (Human); 12096 (Mouse); 25295 (Rat)

Alternate Names

BGLAP; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OC; OCN; Osteocalcin