Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody
Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody Summary
Glu22-Leu401
Accession # AAB53709
Applications
Human Osteoprotegerin/TNFRSF11B Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Osteoprotegerin/TNFRSF11B in MG-63 Human Cell Line. Osteoprotegerin/TNFRSF11B was detected in immersion fixed MG-63 human osteosarcoma cell line using 10 µg/mL Goat Anti-Human Osteoprotegerin/ TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Osteoprotegerin/TNFRSF11B in Human Prostate. Osteoprotegerin/TNFRSF11B was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoprotegerin/TNFRSF11B
Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell‑association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. Human OPG/OCIF cDNA encodes a 401 amino acid (aa) residues precursor protein with a 21 aa residue putative signal peptide that is removed to generate the mature soluble protein. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The 204 residues of the carboxy-terminal OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF kappa B ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1‑4. (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.
Product Datasheets
Citations for Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Severe Dengue Is Associated with Consumption of von Willebrand Factor and Its Cleaving Enzyme ADAMTS-13
Authors: Kis Djamiatun, Andre J. A. M. van der Ven, Philip G. de Groot, Sultana M. H. Faradz, D. Hapsari, Wil M. V. Dolmans et al.
PLoS Neglected Tropical Diseases
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Importance of osteoprotegrin and receptor activator of nuclear factor kappa B in breast cancer response to hepatocyte growth factor and the bone microenvironment in vitro
Authors: SIONED OWEN, ANDREW J. SANDERS, MALCOLM D. MASON, WEN G. JIANG
International Journal of Oncology
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Association between carotid plaque vulnerability and high mobility group box-1 serum levels in a diabetic population
Authors: F Biscetti, G Tinelli, MM Rando, E Nardella, AL Cecchini, F Angelini, G Straface, M Filipponi, V Arena, D Pitocco, A Gasbarrini, M Massetti, A Flex
Cardiovascular Diabetology, 2021-05-27;20(1):114.
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
Serum high mobility group box-1 and osteoprotegerin levels are associated with peripheral arterial disease and critical limb ischemia in type 2 diabetic subjects
Authors: S Giovannini, G Tinelli, F Biscetti, G Straface, F Angelini, D Pitocco, L Mucci, R Landolfi, A Flex
Cardiovasc Diabetol, 2017-08-08;16(1):99.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection) -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
Authors: Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G
Cytokine, 2006-12-11;36(1):57-68.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
J. Rheumatol., 2005-04-01;32(4):713-20.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Dual Therapeutic Action of a Neutralizing Anti-FGF2 Aptamer in Bone Disease and Bone Cancer Pain
Authors: Ling Jin, Yosuke Nonaka, Shin Miyakawa, Masatoshi Fujiwara, Yoshikazu Nakamura
Molecular Therapy
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