Human P-Cadherin Antibody

Detection of Human P‑Cadherin by Western Blot. Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of P-Cadherin in A431 Human Cell Line by Flow Cytometry. A431 human carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

P‑Cadherin in A431 Human Cell Line. P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human P‑Cadherin by Simple Western^TM. Simple Western lane view shows lysates of ZR‑75 human breast cancer cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 135 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human P‑Cadherin Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and P-Cadherin knockout A431 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Cadherin at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

P-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line. P-Cadherin knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

P‑Cadherin Specificity is Shown by Immunocytochemistry in Knockout Cell Line. P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line, wildtype (left panel) but is not detected in P-Cadherin knockout (right panel), using Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane in wildtype cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.