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Human P-Selectin/CD62P Antibody

Catalog #: BBA30
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA30
P-Selectin/CD62P in Human Breast Cancer Tissue.
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Product Details
Citations (9)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P-Selectin/CD62P Antibody Summary

Species Reactivity
Human
Specificity
This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Source
Monoclonal Mouse IgG1 Clone # 9E1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human P-Selectin
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 1 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry P-Selectin/CD62P antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

P-Selectin/CD62P in Human Breast Cancer Tissue. P-Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P-Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

P-Selectin, also known as GMP-140 and PADGEM, is a transmembrane glycoprotein expressed by activated platelets and endothelial cells. P-Selectin binds to PSGL-1 on myeloid cells, neutrophils, monocytes, and lymphocytes. P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

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Citations for Human P-Selectin/CD62P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Platelet-monocyte interaction amplifies thromboinflammation through tissue factor signaling in COVID-19
    Authors: Eugenio D. Hottz, Remy Martins-Gonçalves, Lohanna Palhinha, Isaclaudia G. Azevedo-Quintanilha, Mariana M. de Campos, Carolina Q. Sacramento et al.
    Blood Advances
  2. SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation
    Authors: Luca Perico, Marina Morigi, Miriam Galbusera, Anna Pezzotta, Sara Gastoldi, Barbara Imberti et al.
    Frontiers in Immunology
  3. Maternal, Fetal, and Placental Selectins in Women With Pre-eclampsia; Association With the Renin-Angiotensin-System
    Authors: Hiten D. Mistry, Melissa V. Hott Ogalde, Fiona Broughton Pipkin, Geneviève Escher, Lesia O. Kurlak
    Frontiers in Medicine
  4. Adhesion of T cells to endothelial cells facilitates blinatumomab-associated neurologic adverse events
    Authors: M Klinger, G Zugmaier, V Nägele, ME Goebeler, C Brandl, M Stelljes, H Lassmann, A von Stacke, RC Bargou, P Kufer
    Cancer Res., 2019-10-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue.
    Authors: Hottz E, Medeiros-de-Moraes I, Vieira-de-Abreu A, de Assis E, Vals-de-Souza R, Castro-Faria-Neto H, Weyrich A, Zimmerman G, Bozza F, Bozza P
    J Immunol, 2014-07-11;193(4):1864-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
    Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
    J Immunol, 2014-04-11;192(10):4674-84.
    Species: Human
    Sample Types: Whole Blood
    Applications: Neutralization
  7. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006-06-15;298(1):107-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. P-Selectin-mediated acute inflammation can be blocked by chemically modified heparin, RO-heparin.
    Authors: Gao Y, Li N, Fei R, Chen Z, Zheng S, Zeng X
    Mol. Cells, 2005-06-30;19(3):350-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Platelets mediate inflammatory monocyte activation by SARS-CoV-2 Spike protein
    Authors: Li T, Yang Y, Li Y et al.
    The Journal of clinical investigation

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Reviews for Human P-Selectin/CD62P Antibody

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Human P-Selectin/CD62P Antibody
By Balaji Mahender on 12/20/2017
Application: ELISA Sample Tested: Citrate Plasma,EDTA Plasma,Heparin Plasma Species: Human