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Human P-Selectin/CD62P Antibody

Catalog #: BBA32
3 Citations Datasheet / COA / SDS
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BBA32
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Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P-Selectin/CD62P Antibody Summary

Species Reactivity
Human
Specificity
Selected for its ability to inhibit the binding of U937 cells to immobilized rhP-Selectin. In Western blots, less than 1% cross-reactivity is observed with rhE‑Selectin and rhL‑Selectin.
Source
Polyclonal Sheep IgG
Purification
Protein A or G purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human P-Selectin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human P-Selectin/CD62P (Catalog # ADP3)
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized recombinant human P‑Selectin (10 μg/mL, 100 µL/well) was maximally inhibited (80 - 100%) by 25 μg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute with sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

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Citations for Human P-Selectin/CD62P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport
    Authors: Mafalda Lopes-da-Silva, Jessica J. McCormack, Jemima J. Burden, Kimberly J. Harrison-Lavoie, Francesco Ferraro, Daniel F. Cutler
    Developmental Cell
  2. Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway.
    Authors: Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC
    Circ. Res., 2007-09-20;101(10):1001-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Blockade
  3. TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
    Authors: Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
    Atherosclerosis, 2004-12-23;179(1):51-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: Neutralization

FAQs

  1. Does Human P-Selectin/CD62P Antibody, Catalog # BBA32, work under both reducing and non-reducing conditions in Western Blot?

    • We have tested BBA32 in Western Blot against recombinant Human P-Selectin, catalog # ADP3, and observe bands under both reducing and non-reducing conditions.

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