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Human P-Selectin/CD62P ELISA Kit - Quantikine

Catalog #: DPSE00
18 Citations 2 Reviews Datasheet / COA / SDS
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Human P-Selectin ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human P-Selectin/CD62P Quantikine ELISA Kit Summary

Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
0.121 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Rrecombinant and natural human P-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human P-Selectin in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=33)82.643.5-11920.0
EDTA plasma (n=33)35.318.3-57.48.78
Heparin plasma (n=33)33.718.6-50.67.40

Sample TypeMean of Detectable (ng/mL)% DetectableRange (ng/mL)
Citrate plasma (n=33)25.897ND-37.9
ND=Non-detectable


Product Summary

The Quantikine® Human P-Selectin/CD62P Immunoassay is a 1.25 hour solid-phase ELISA designed to measure human P-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human P-Selectin and antibodies raised against the recombinant factor. Results obtained using natural human P-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human P-Selectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 6.54 19.3 25.6 6.51 19.9 30.5
Standard Deviation 0.442 1.47 2.29 0.484 1.57 3.64
CV% 6.8 7.6 8.9 7.4 7.9 11.9

Recovery

The recovery of Human P-Selectin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 88-107
Citrate Plasma (n=4) 92 81-111
EDTA Plasma (n=4) 92 77-103
Heparin Plasma (n=4) 95 78-112
Serum (n=4) 93 77-104

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human P-Selectin were diluted with sample diluent to produce samples with values within the dynamic range of the assay.
Human P-Selectin Linearity

Scientific Data

N/A Human P-Selectin ELISA Standard Curve View Larger

Human P-Selectin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: P-Selectin/CD62P

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Entrez Gene IDs:
6403 (Human); 20344 (Mouse)
Alternate Names:
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human P-Selectin/CD62P Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Surface topography modulates initial platelet adhesion to titanium substrata
    Authors: Guo, CY;Mo, R;Kim, H;
    Journal of oral biology and craniofacial research
    Species: Human
    Sample Types: Platelet Supernates
  2. Low-grade endotoxemia in acute pulmonary embolism: Links with prothrombotic plasma fibrin clot phenotype
    Authors: Z?bczyk, M;Kruk, A;Natorska, J;Undas, A;
    Thrombosis research
    Species: Human
    Sample Types: Serum
  3. Technically Challenging Percutaneous Interventions of Chronic Total Occlusions Are Associated with Enhanced Platelet Activation
    Authors: Illési, Á;Fejes, Z;Pócsi, M;Debreceni, IB;Hodosi, K;Nagy, B;Kappelmayer, J;K?szegi, Z;Csanádi, Z;Szük, T;
    Journal of clinical medicine
  4. Increasing Soluble P-Selectin Levels Predict Higher Peripheral Atherosclerotic Plaque Progression
    Authors: Sommer, P;Schreinlechner, M;Noflatscher, M;Lener, D;Mair, F;Theurl, M;Kirchmair, R;Marschang, P;
    Journal of clinical medicine
    Species: Human
    Sample Types: Plasma
  5. Association of ADP-Induced Whole-Blood Platelet Aggregation with Serum Low-Density Lipoprotein Cholesterol in Patients with Coronary Artery Disease When Receiving Maintenance Ticagrelor-Based Dual Antiplatelet Therapy
    Authors: Chyrchel, B;Kruszelnicka, O;Wieczorek-Surdacka, E;Surdacki, A;
    Journal of clinical medicine
    Species: Human
    Sample Types: Plasma
  6. Usefulness of blood biomarkers for predicting venous thromboembolism in Japanese patients with cancer
    Authors: Y Harada, A Sato, A Nishioka, S Ogusu, M Matsumoto, E Sueoka, A Kawaguchi, S Kimura, N Sueoka-Ara
    Oncology Letters, 2023-03-17;25(5):180.
    Species: Human
    Sample Types: Plasma
  7. Platelet function and soluble P-selectin in patients with primary immune thrombocytopenia
    Authors: D Mehic, J Machacek, T Schramm, L Buresch, A Kaider, B Eichelberg, H Haslacher, M Fillitz, B Dixer, T Flasch, T Anderle, A Rath, A Assinger, C Ay, I Pabinger, J Gebhart
    Thrombosis Research, 2023-01-16;223(0):102-110.
    Species: Human
    Sample Types: Serum
  8. Early but reversible haemostatic changes in a-symptomatic females expressing COVID-19 antibodies
    Authors: JO Williams, J Nash, C Whelan, BM Raven, AJ Davies, J Evans, L Watkeys, K Morris, PE James
    Thrombosis Research, 2022-07-25;217(0):76-85.
    Species: Human
    Sample Types: Plasma
  9. Short-Term Treatment with Alirocumab, Flow-Dependent Dilatation of the Brachial Artery and Use of Magnetic Resonance Diffusion Tensor Imaging to Evaluate Vascular Structure: An Exploratory Pilot Study
    Authors: T Metzner, DR Leitner, G Dimsity, F Gunzer, P Opriessnig, K Mellitzer, A Beck, H Sourij, T Stojakovic, H Deutschman, W März, U Landmesser, M Brodmann, G Reishofer, H Scharnagl, H Toplak, G Silbernage
    Biomedicines, 2022-01-11;10(1):.
    Species: Human
    Sample Types: Plasma
  10. Controlled sulfation of mixed-linkage glucan by Response Surface Methodology for the development of biologically applicable polysaccharides
    Authors: L Solhi, HS Sun, SH Daswani, S Shojania, CMK Springate, H Brumer
    Carbohydrate polymers, 2021-06-01;269(0):118275.
    Species: Human
    Sample Types: Protein
  11. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
    Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
    Nature Communications, 2021-03-26;12(1):1912.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Biomarker-enhanced VTE risk stratification in ambulatory patients with cancer
    Authors: JR Shaw, V Kumar, R Mallick, M Carrier, A Ilich, NS Key, P Wells
    Thromb Res, 2020-10-08;196(0):437-443.
    Species: Human
    Sample Types: Plasma
  13. Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures
    Authors: M Mussbacher, TL Krammer, S Heber, WC Schrottmai, S Zeibig, HP Holthoff, D Pereyra, P Starlinger, M Hackl, A Assinger
    Cells, 2020-08-18;9(8):.
    Species: Human
    Sample Types: Plasma
  14. Elevated Lactate Levels in Acute Pulmonary Embolism Are Associated with Prothrombotic Fibrin Clot Properties: Contribution of NETs Formation
    Authors: M Z?bczyk, J Natorska, A Janion-Sad, KP Malinowski, M Janion, A Undas
    J Clin Med, 2020-03-30;9(4):.
    Species: Human
    Sample Types: Plasma
  15. The Ser290Asn and Thr715Pro Polymorphisms of the SELP Gene Are Associated with A Lower Risk of Developing Acute Coronary Syndrome and Low Soluble P-Selectin Levels in A Mexican Population �
    Authors: G Herrera-Ma, G Vargas-Ala, O Pérez-Ménd, R Posadas-Sá, F Masso, T Juárez-Ced, G Escobedo, A Vázquez-Mo, JM Fragoso
    Biomolecules, 2020-02-11;10(2):.
    Species: Human
    Sample Types: Plasma
  16. Amaranth Oil Increases Total and LDL Cholesterol Levels without Influencing Early Markers of Atherosclerosis in an Overweight and Obese Population: A Randomized Double-Blind Cross-Over Study in Comparison with Rapeseed Oil Supplementation
    Authors: M Dus-Zuchow, J Walkowiak, A Morawska, P Krzyzanows, A Miskiewicz, J Przyslawsk, A Lisowska
    Nutrients, 2019-12-16;11(12):.
    Species: Human
    Sample Types: Plasma
  17. Predicting the risk of recurrent venous thromboembolism in patients with cancer: A prospective cohort study
    Authors: N van Es, M Louzada, M Carrier, V Tagalakis, PL Gross, S Shivakumar, MA Rodger, PS Wells
    Thromb. Res., 2018-01-09;163(0):41-46.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  18. Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
    Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
    Iran J Pathol, 2017-01-27;12(3):257-264.
    Species: Human
    Sample Types: Serum

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Human P-Selectin/CD62P Quantikine ELISA Kit
By Anonymous on 10/12/2021
Sample Tested: Citrate Plasma

We diluted the plasma 1:5

Human P-Selectin/CD62P Quantikine ELISA Kit DPSE00
Human P-Selectin/CD62P Quantikine ELISA Kit
By Anonymous on 05/27/2019
Sample Tested: EDTA Plasma
Human P-Selectin/CD62P Quantikine ELISA Kit DPSE00