Human P2Y10/P2RY10 Antibody

Catalog #: MAB10158
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10158-100
MAB10158-SP

Detection of P2Y10/P2RY10 in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow Cytometry.

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All P2Y10/P2RY10 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P2Y10/P2RY10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human P2RY10 in Flow Cytometry and Immunohistochemistry.

Source

Monoclonal Mouse IgG_2B Clone # 639329

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human P2RY10
Accession # O00398

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of P2Y10/P2RY10 in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with P2Y10/P2RY10 and eGFP was stained with (A) Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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P2Y10/P2RY10 in Human Thymus. P2Y10/P2RY10 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2Y10/P2RY10

P2Y receptors are a family of purinergic G protein-coupled receptors which belong to the class A family of GPCRs. They are membrane proteins, preferentially activated by nucleotides such as ADP, ATP, UTP, UDP and UDP-glucose. This P2Y receptor is thought to be a receptor for purines. While there is little known about the activity of this specific receptor, P2Y receptors mediate various biological functions such as vasodilation, blood clotting and immune responses.

Long Name

P2Y Purinergic Receptor 10

Entrez Gene IDs

27334 (Human); 78826 (Mouse); 317219 (Rat)

Alternate Names

G-protein coupled purinergic receptor P2Y10; P2RY10; P2Y purinoceptor 10; P2Y10; P2Y10P2Y-like receptor; purinergic receptor P2Y, G-protein coupled, 10; putative P2Y purinoceptor 10

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human P2Y10/P2RY10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1