Home / p63/TP73L / Human p63/TP73L Biotinylated Antibody

Human p63/TP73L Biotinylated Antibody

Catalog #: BAF1916
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF1916
p63/TP73L in Human Prostate.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p63/TP73L Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects p63/TP73L in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p63/TP73L
Met40-Cys339
Accession # Q9H3D4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human p63/TP73L
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry p63/TP73L antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

p63/TP73L in Human Prostate. p63/TP73L was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry p63/TP73L antibody in SCC-25 Human Cell Line by Immunocytochemistry (ICC). View Larger

p63/TP73L in SCC-25 Human Cell Line. p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

References
  1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
  2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
  3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.
Long Name
Tumor Protein 63
Entrez Gene IDs
8626 (Human)
Alternate Names
amplified in squamous cell carcinoma; Chronic ulcerative stomatitis protein; CUSP; EEC3; EEC3B(p51B); Keratinocyte transcription factor KET; KET; KETRHS; LMS; OFC8; OFC8SHFM4TP73LNBP; p40; p51; p51tumor protein p53-like; p53CP; p63; p63AIS; p73H; p73L; p73LB(p51A); SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; tumor protein p63 deltaN isoform delta; tumor protein p63; Tumor protein p73-like

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Citations for Human p63/TP73L Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Retinoic acid receptor gamma activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium
    Authors: Yasufumi Koterazawa, Michiyo Koyanagi-Aoi, Keiichiro Uehara, Yoshihiro Kakeji, Takashi Aoi
    Journal of Gastroenterology
  2. R-SPONDIN2+ mesenchymal cells form the bud tip progenitor niche during human lung development
    Authors: Renee F.C. Hein, Joshua H. Wu, Emily M. Holloway, Tristan Frum, Ansley S. Conchola, Yu-Hwai Tsai et al.
    Developmental Cell
  3. In Vitro and In Vivo Development of the Human Airway at Single-Cell Resolution
    Authors: Alyssa J. Miller, Qianhui Yu, Michael Czerwinski, Yu-Hwai Tsai, Renee F. Conway, Angeline Wu et al.
    Developmental Cell
  4. Opposing roles for TGF?- and BMP-signaling during nascent alveolar differentiation in the developing human lung
    Authors: Frum, T;Hsu, PP;Hein, RFC;Conchola, AS;Zhang, CJ;Utter, OR;Anand, A;Zhang, Y;Clark, SG;Glass, I;Sexton, JZ;Spence, JR;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  5. Generation of lung organoids from human pluripotent stem cells in vitro
    Authors: AJ Miller, BR Dye, D Ferrer-Tor, DR Hill, AW Overeem, LD Shea, JR Spence
    Nat Protoc, 2019-02-01;14(2):518-540.

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