Human Pappalysin-2/PAPP-A2 Biotinylated Antibody

Catalog #: BAF1668 Datasheet / COA / SDS

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BAF1668

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pappalysin-2/PAPP-A2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pappalysin‑2/PAPP‑A2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant humah PAPP-A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
Ser234-Cys1396
Accession # Q9BXP8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pappalysin-2/PAPP-A2

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.

References

Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
Boldt, H.B. et al. (2001) Biochem. J. 358:359.
Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
Laursen, L.S. et al. (2001) FEBS Lett. 504:36.

Long Name

Pregnancy-associated Plasma Protein

Entrez Gene IDs

60676 (Human)

Alternate Names

EC 3.4.24.-; PAPPA2; PAPP-A2; PAPP-A2pappalysin-2; pappalysin 2; Pappalysin2; Pappalysin-2; PAPPE; PAPP-E; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; Pregnancy-associated plasma protein A2; Pregnancy-associated plasma protein E1