Human PAR1 Alexa Fluor® 488-conjugated Antibody Summary
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3855G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of PAR1 in Human Peripheral Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 APC-conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human PAR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3855G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR1
Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human PAR1 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Characterization of Biomarkers of Thrombo-Inflammation in Patients with First-Diagnosed Atrial Fibrillation
Authors: Friebel, J;Wegner, M;Blöbaum, L;Schencke, PA;Jakobs, K;Puccini, M;Ghanbari, E;Lammel, S;Thevathasan, T;Moos, V;Witkowski, M;Landmesser, U;Rauch-Kröhnert, U;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function
Authors: Y Roka-Moiia, KR Ammann, S Miller-Gut, J Sheriff, D Bluestein, JE Italiano, RC Flaumenhaf, MJ Slepian
International Journal of Molecular Sciences, 2023-04-17;24(8):.
Species: Human
Sample Types: Complex Sample Type
Applications: Flow Cytometry -
Cytotoxic CD8+ T Cells Are Involved in the Thrombo-Inflammatory Response during First-Diagnosed Atrial Fibrillation
Authors: J Friebel, M Witkowski, M Wegner, L Blöbaum, S Lammel, PA Schencke, K Jakobs, M Puccini, D Rei beta ner, D Steffens, V Moos, HP Schutheiss, U Landmesser, U Rauch
Cells, 2022-12-29;12(1):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption
Authors: M Avril, M Benjamin, MM Dols, JD Smith
Sci Rep, 2019-09-11;9(1):13142.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization.
Authors: Alejandro Alvarez-Ar, Irene Lee-River, Edith López, Arturo Hernández, Ana María López-Col
International Journal of Cell Biology, 2017-11-09;0(0):1687-8876.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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