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Human PAR2 Antibody

Catalog #: MAB3949
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3949
MAB3949-SP
Detection of PAR2 in HT‑29 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PAR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 344222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HT‑29 human colon adenocarcinoma cell line and PC-3 prostate cancer cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PAR2 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PAR2 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B).

Flow Cytometry View Larger

Detection of PAR2 in PC-3 cells by Flow Cytometry. PC-3 cells were stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAR2

Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.

Long Name
Protease-Activated Receptor 2
Entrez Gene IDs
2150 (Human); 14063 (Mouse); 116677 (Rat)
Alternate Names
CFIIRL1; coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2RL1; GPCR11; GPR11protease-activated receptor 2; G-protein coupled receptor 11; PAR2; PAR-2; PAR2G protein-coupled receptor-11; proteinase-activated receptor 2; proteinase-activated receptor-2; Thrombin receptor-like 1

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Citations for Human PAR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Structural insight into allosteric modulation of protease-activated receptor 2
    Authors: RKY Cheng, C Fiez-Vanda, O Schlenker, K Edman, B Aggeler, DG Brown, GA Brown, RM Cooke, CE Dumelin, AS Doré, S Geschwindn, C Grebner, NO Hermansson, A Jazayeri, P Johansson, L Leong, R Prihandoko, M Rappas, H Soutter, A Snijder, L Sundström, B Tehan, P Thornton, D Troast, G Wiggin, A Zhukov, FH Marshall, N Dekker
    Nature, 2017-04-26;545(7652):112-115.
    Species: Human, N/A
    Sample Types: Protein, Whole Cells
    Applications: Binding Model, Neutralization
  2. Transient Photo-Inactivation of Cell Membrane Protein Activity without Genetic Modification by Molecular Hyperthermia
    Authors: Peiyuan Kang, Xiaoqing Li, Yaning Liu, Stephanie I. Shiers, Hejian Xiong, Monica Giannotta et al.
    ACS Nano

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