Human PAR2 APC-conjugated Antibody

Catalog #: FAB3949A Datasheet / COA / SDS

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FAB3949A

Detection of PAR2 in HT-29 Human Cell Line by Flow Cytometry.

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Human PAR2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 344222

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of PAR2 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 APC-conjugated Monoclonal Antibody (Catalog # FAB3949A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: PAR2

Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.

Long Name

Protease-Activated Receptor 2

Entrez Gene IDs

2150 (Human); 14063 (Mouse); 116677 (Rat)

Alternate Names

CFIIRL1; coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2RL1; GPCR11; GPR11protease-activated receptor 2; G-protein coupled receptor 11; PAR2; PAR-2; PAR2G protein-coupled receptor-11; proteinase-activated receptor 2; proteinase-activated receptor-2; Thrombin receptor-like 1