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Human PAR2 PE-conjugated Antibody

Catalog #: FAB3949P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3949P
Detection of PAR2 in HT‑29 Human Colon Adeno-carcinoma Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PAR2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 344222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
HT‑29 human colon adenocarcinoma cell line or PC-3 prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PAR2 antibody in HT-29 Human Colon Adeno-carcinoma Cell Line antibody by Flow Cytometry. View Larger

Detection of PAR2 in HT‑29 Human Colon Adeno-carcinoma Cell Line by Flow Cytometry. HT-29 human colon adeno-carcinoma cell line was stained with Mouse Anti-Human PAR2 PE-conjugated Mono-clonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of PAR2 in PC-3 cells by Flow Cytometry. PC-3 cells were stained with Mouse Anti-Human PAR2 PE‑conjugated Monoclonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PAR2

Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.

Long Name
Protease-Activated Receptor 2
Entrez Gene IDs
2150 (Human); 14063 (Mouse); 116677 (Rat)
Alternate Names
CFIIRL1; coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2RL1; GPCR11; GPR11protease-activated receptor 2; G-protein coupled receptor 11; PAR2; PAR-2; PAR2G protein-coupled receptor-11; proteinase-activated receptor 2; proteinase-activated receptor-2; Thrombin receptor-like 1

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Citations for Human PAR2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mast cell-derived tryptase inhibits apoptosis of human rheumatoid synovial fibroblasts via rho-mediated signaling.
    Authors: Sawamukai N, Yukawa S, Saito K
    Arthritis Rheum., 2010-04-01;62(4):952-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts.
    Authors: Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C
    Bone, 2009-03-02;44(6):1143-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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