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Human PARD3/Par3 Antibody

Catalog #: MAB8030
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8030
MAB8030-SP
PARD3/Par3 in HEK293 Human Cell Line.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PARD3/Par3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PARD3/Par3 in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.
Source
Monoclonal Mouse IgG1 Clone # 833517
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PARD3/Par3
Tyr451-Glu555
Accession # Q8TEW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry PARD3/Par3 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

PARD3/Par3 in HEK293 Human Cell Line. PARD3/Par3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human PARD3/Par3 Monoclonal Antibody (Catalog # MAB8030) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PARD3/Par3

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Long Name
Par-3 Partitioning Defective 3 Homolog
Entrez Gene IDs
56288 (Human); 93742 (Mouse); 81918 (Rat)
Alternate Names
ASIP; Baz; Bazooka; FLJ21015; par-3 partitioning defective 3 homolog (C. elegans); PAR3; PAR3A; PAR3C.elegans) homolog; PARD3; SE2-5L16; SE2-5LT1; SE2-5T2

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Citations for Human PARD3/Par3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. DAPLE orchestrates apical actomyosin assembly from junctional polarity complexes
    Authors: Arthur Marivin, Rachel Xi-Yeen Ho, Mikel Garcia-Marcos
    Journal of Cell Biology
  2. Hypoxia disrupt tight junctions and promote metastasis of oral squamous cell carcinoma via loss of par3
    Authors: S Kim, S Park, EH Moon, GJ Kim, J Choi
    Cancer Cell International, 2023-04-24;23(1):79.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissues
    Applications: Western Blot, ICC, IHC

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