Human Patched 2/PTCH2 (First Extracellular Loop) Antibody

Catalog #: AF4078 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4078
AF4078-SP

Detection of Patched 2 in WS‑1 Human Cell Line by Flow Cytometry.

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Human Patched 2/PTCH2 (First Extracellular Loop) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Patched 2/PTCH2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Patched is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Patched 2/PTCH2 first extracellular loop
Glu79-Glu392
Accession # Q9Y6C5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Patched 2 (First Extracellular Loop)

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Patched 2 antibody in WS‑1 Human Cell Line antibody by Flow Cytometry.

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Detection of Patched 2 in WS‑1 Human Cell Line by Flow Cytometry. WS‑1 human fetal skin fibroblast cell line was stained with Human Patched 2 (First Extracellular Loop) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4078, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunohistochemistry

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Patched 2 in Human Basal Cell Carcinoma. Patched 2 was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using 3 µg/mL Goat Anti-Human Patched 2 (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4078) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 2/PTCH2

Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. Human PTCH2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa 1115-1203. Together with Smoothened (SMO), Ptch2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Human Ptch2 likely has two isoforms. One shows an eight aa extension of the C-terminus, while a second shows a four aa substitution for the C-terminal 61 amino acids (aa 1143-1203). Over the first extracellular loop human Patched 2 shares 95% and 96% aa sequence identity with mouse and canine Patched 2, respectively.

Entrez Gene IDs

8643 (Human); 19207 (Mouse)

Alternate Names

patched (Drosophila) homolog 2; Patched 2; patched homolog 2 (Drosophila); patched homolog 2; protein patched homolog 2; ptc2; PTCH2