Human PCK1 Antibody

Catalog #: MAB7316 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7316
MAB7316-SP

Detection of Human PCK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PCK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PCK1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 789114

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PCK1
Met1-Ile88
Accession # P35558

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PCK1 by Western Blot. Western blot shows lysates of human liver tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PCK1 Monoclonal Antibody (Catalog # MAB7316) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PCK1

PCK1 (Phosphoenolpyruvate carboxykinase 1; also PEPCK-C [cytosolic]) is a monomeric, 67-68 kDa member of the PEP carboxykinase family of enzymes. It is expressed in postnatal cells such as mammary epithelium, white and brown adipocytes, skeletal muscle cells and hepatocytes. PCK1 has multiple functions, some of which are cell-specific. In particular, PCK1 has both cataplerotic (Greek: to fill down, or remove) and anaplerotic (to fill up, or replace) activity, where it removes and replaces elements of the TCA cycle. It is also gluconeogenic, and promotes glucose formation via PEP generation. Finally, it is glyceroneogenic, creating glycerol-3-phosphate that is used to reesterify and store just-released free fatty acids in adipocytes. Mouse PCK1 is 622 amino acids (aa) in length. It contains one kinase domain (aa 27-615), and two potential acetylation sites at Lys70 and 71. There are four potential splice forms. Two have alternative start sites at Met460 and Met315, while two others show a deletion of aa 34-546, plus a three aa substitution for aa 85-204, respectively. Over aa 551-622, mouse PCK1 shares 93% and 82% aa identity with rat and human PCK1, respectively.

Long Name

Phosphoenolpyruvate Carboxykinase 1

Entrez Gene IDs

5105 (Human); 18534 (Mouse); 362282 (Rat)

Alternate Names

EC 4.1.1.32; MGC22652; PCK1; PEPCK1; PEPCKC; PEPCK-C; PEPCK-CPEP carboxykinase; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic; Phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase