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Human PCPE-1 Antibody

Catalog #: AF2627
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2627
AF2627-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PCPE-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PCPE-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PCPE-1
Met1-Asp449
Accession # Q15113
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PCPE‑1
Immunoprecipitation
25 µg/mL
Conditioned cell culture media from NS0 cells transfected with recombinant human PCPE-1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PCPE-1

PCPE-1 is a 55 kDa extracellular protein that lacks intrinsic enzymatic activity. It functions as a substrate-specific adaptor protein that stimulates C-terminal processing of the fibrillar procollagens I, II and III by PCP (also known as BMP-1/tolloid metalloproteinases). PCPE is naturally processed into N-terminal fragments of 34 and 36 kDas, both of which also enhance PCP. A 16.5 kDa protein corresponding to a C-terminal fragment of PCPE has been described and shown to have metalloproteinase inhibitor activity. Human PCPE-1 shares approximately 81% amino acid sequence homology with mouse and rat PCPE-1.

Long Name
Procollagen C-Proteinase Enhancer-1
Entrez Gene IDs
5118 (Human); 18542 (Mouse)
Alternate Names
PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1Type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; Procollagen C-proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein

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Citation for Human PCPE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of PCPE-2 as the endogenous specific inhibitor of human BMP-1/tolloid-like proteinases
    Authors: Vadon-Le Goff, S;Tessier, A;Napoli, M;Dieryckx, C;Bauer, J;Dussoyer, M;Lagoutte, P;Peyronnel, C;Essayan, L;Kleiser, S;Tueni, N;Bettler, E;Mariano, N;Errazuriz-Cerda, E;Fruchart Gaillard, C;Ruggiero, F;Becker-Pauly, C;Allain, JM;Bruckner-Tuderman, L;Nyström, A;Moali, C;
    Nature communications
    Species: Mouse, Human
    Sample Types: Protein
    Applications: Western Blot

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