Human PD-ECGF/Thymidine Phosphorylase Antibody

Catalog #: AB-229-NA Datasheet / COA / SDS

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AB-229-NA

Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.

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All PD-ECGF/Thymidine Phosphorylase Antibodies

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Human PD-ECGF/Thymidine Phosphorylase Antibody Summary

Species Reactivity

Human

Specificity

Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with ____________ is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD-ECGF/Thymidine Phosphorylase
Ala11-Gln482
Accession # P19971.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human PD-ECGF (Catalog # 229-PE)

Neutralization

Measured by its ability to neutralize PD‑ECGF-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND₅₀) is typically 10-25 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody. Recombinant Human PD-ECGF/Thymidine Phosphorylase (Catalog # 229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND₅₀ is typically 10-25 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-ECGF/Thymidine Phosphorylase

PD-ECGF, also known as TPase and gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5-fluorouracil and its derivatives. The human PD-ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The latter protein was purified from quiescent astrocytes and described as an astrocyte and astrocytoma cell growth inhibitor based on its ability to inhibit ³H-thymidine incorporation.

References

Haraguchi, M. et al. (1994) Nature 368:198.
Toi, M. et al. (2005) Lancet Oncol. 6:158.
Akiyama, S. et al. (2004) Canc. Sci. 95:851.

Long Name

Platelet-derived Endothelial Cell Growth Factor

Entrez Gene IDs

1890 (Human); 72962 (Mouse)

Alternate Names

dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP