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Human PD-L1/B7-H1 Antibody

Catalog #: MAB1561
24 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1561
MAB1561-SP
Detection of PD-L1/B7-H1 in Jurkat Human Cell Line by Flow Cytometry.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PD-L1/B7-H1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PD-L1/B7-H1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, -2, -H2, -H3, -H3b,
-H4, rhPD-L2, recombinant mouse B7-H1, recombinant rat (rr) B7-1, or rrB7-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 130021
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1
Phe19-Thr239
Accession # Q9NZQ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human colon cancer

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PD-L1/B7-H1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of PD-L1/B7-H1 antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry PD-L1/B7-H1 antibody in Human Colon Cancer by Immunohistochemistry (IHC-P). View Larger

PD-L1/B7-H1 in Human Colon Cancer. PD-L1/B7-H1 was detected in formalin fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was observed in the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

In-situ Hybridization View Larger

Detection of PD-L1/B7-H1 in Human Colon Cancer. Formalin-fixed paraffin-embedded tissue sections of human colon cancer were probed for PDL1 mRNA (ACD RNAScope Probe, catalog # 600861; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human PDL1 monoclonal antibody (R&D Systems catalog # MAB1561) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. Their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, there is about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 291 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

References
  1. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  2. Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027.
  3. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

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Citations for Human PD-L1/B7-H1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Potential immune escape mechanisms underlying the distinct clinical outcome of immune checkpoint blockades in small cell lung cancer
    Authors: Y Tian, X Zhai, A Han, H Zhu, J Yu
    J Hematol Oncol, 2019-06-28;12(1):67.
  2. Efficacy and safety of camrelizumab (a PD-1 inhibitor) combined with chemotherapy as a neoadjuvant regimen in patients with locally advanced non-small cell lung cancer
    Authors: X Hou, X Shi, J Luo
    Oncology Letters, 2022-05-17;24(1):215.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Proteomic Alterations and Novel Markers of Neurotoxic Reactive Astrocytes in Human Induced Pluripotent Stem Cell Models
    Authors: David Labib, Zhen Wang, Priya Prakash, Matthew Zimmer, Matthew D. Smith, Paul W. Frazel et al.
    Frontiers in Molecular Neuroscience
  4. FOXO3-dependent suppression of PD-L1 promotes anticancer immune responses via activation of natural killer cells
    Authors: YM Chung, WB Tsai, PP Khan, J Ma, JS Berek, JW Larrick, MC Hu
    American journal of cancer research, 2022-03-15;12(3):1241-1263.
    Species: Human
    Sample Types: Whole Cells
    Applications: IF
  5. Sensitizing tumors to anti-PD-1 therapy by promoting NK and CD8+ T cells via pharmacological activation of FOXO3
    Authors: Young Min Chung, Pragya P Khan, Hong Wang, Wen-Bin Tsai, Yanli Qiao, Bo Yu et al.
    Journal for ImmunoTherapy of Cancer
  6. Adipose-Tissue-Derived Mesenchymal Stem Cells Mediate PD-L1 Overexpression in the White Adipose Tissue of Obese Individuals, Resulting in T Cell Dysfunction
    Authors: A Eljaafari, J Pestel, B Le Maguere, S Chanon, J Watson, M Robert, E Disse, H Vidal
    Cells, 2021-10-03;10(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Radiotherapy Combined with PD-1 Inhibition Increases NK Cell Cytotoxicity towards Nasopharyngeal Carcinoma Cells
    Authors: A Makowska, N Lelabi, C Nothbaum, L Shen, P Busson, TTB Tran, M Eble, U Kontny
    Cells, 2021-09-17;10(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. ImmunoCluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry data
    Authors: James W Opzoomer, Jessica A Timms, Kevin Blighe, Thanos P Mourikis, Nicolas Chapuis, Richard Bekoe et al.
    eLife
  9. Serum levels of soluble programmed death-ligand 1 (sPD-L1): A possible biomarker in predicting post-treatment outcomes in patients with early hepatocellular carcinoma
    Authors: T Mocan, M Ilies, I Nenu, R Craciun, A Horhat, R Susa, I Minciuna, I Rusu, LP Mocan, A Seicean, CA Iuga, NA Hajjar, M Sparchez, DC Leucuta, Z Sparchez
    International immunopharmacology, 2021-02-18;94(0):107467.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Tumour PD-L1 Expression in Small-Cell Lung Cancer: A Systematic Review and Meta-Analysis
    Authors: E Acheampong, A Abed, M Morici, S Bowyer, B Amanuel, W Lin, M Millward, ES Gray
    Cells, 2020-10-31;9(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Dissecting the immunosuppressive tumor microenvironments in Glioblastoma-on-a-Chip for optimized PD-1 immunotherapy
    Authors: Xin Cui, Chao Ma, Varshini Vasudevaraja, Jonathan Serrano, Jie Tong, Yansong Peng et al.
    eLife
  12. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019-05-02;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  13. Accumulation of circulating CCR7+ natural killer cells marks melanoma evolution and reveals a CCL19-dependent metastatic pathway
    Authors: CM Cristiani, A Turdo, V Ventura, T Apuzzo, M Capone, G Madonna, D Mallardo, C Garofalo, ED Giovannone, AM Grimaldi, R Tallerico, E Marcenaro, S Pesce, G Del Zotto, V Agosti, FS Costanzo, E Gulletta, A Rizzo, A Moretta, K Kärre, PA Ascierto, M Todaro, E Carbone
    Cancer Immunol Res, 2019-04-02;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  14. Viral Load Affects the Immune Response to HBV in Mice With Humanized Immune System and Liver
    Authors: Mathilde Dusséaux, Guillemette Masse-Ranson, Sylvie Darche, James Ahodantin, Yan Li, Oriane Fiquet et al.
    Gastroenterology
  15. Hilar fat infiltration: A new prognostic factor in metastatic clear cell renal cell carcinoma with first-line sunitinib treatment
    Authors: SF Kammerer-J, A Brunot, K Bensalah, B Campillo-G, M Lefort, S Bayat, A Ravaud, F Dupuis, M Yacoub, G Verhoest, B Peyronnet, R Mathieu, A Lespagnol, J Mosser, J Edeline, B Laguerre, JC Bernhard, N Rioux-Lecl
    Urol. Oncol., 2017-06-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  16. Soluble PD-L1 as a biomarker in malignant melanoma and checkpoint blockade
    Authors: J Zhou, KM Mahoney, A Giobbie-Hu, F Zhao, S Lee, X Liao, S Rodig, J Li, X Wu, LH Butterfiel, M Piesche, MP Manos, LM Eastman, G Dranoff, GJ Freeman, FS Hodi
    Cancer Immunol Res, 2017-05-18;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  17. An Immune Atlas of Clear Cell Renal Cell Carcinoma
    Authors: Stéphane Chevrier, Jacob Harrison Levine, Vito Riccardo Tomaso Zanotelli, Karina Silina, Daniel Schulz, Marina Bacac et al.
    Cell
  18. Development of a programmed cell death ligand-1 immunohistochemical assay validated for analysis of non-small cell lung cancer and head and neck squamous cell carcinoma
    Authors: Marlon C. Rebelatto, Anita Midha, Amita Mistry, Constantine Sabalos, Nicole Schechter, Xia Li et al.
    Diagnostic Pathology
  19. Independent association of PD-L1 expression with noninactivated VHL clear cell renal cell carcinoma-A finding with therapeutic potential.
    Authors: Kammerer-Jacquet S, Crouzet L, Brunot A, Dagher J, Pladys A, Edeline J, Laguerre B, Peyronnet B, Mathieu R, Verhoest G, Patard J, Lespagnol A, Mosser J, Denis M, Messai Y, Gad-Lapiteau S, Chouaib S, Belaud-Rotureau M, Bensalah K, Rioux-Leclercq N
    Int J Cancer, 2016-09-23;140(1):142-148.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Frequent expression of PD-L1 on circulating breast cancer cells.
    Authors: Mazel M, Jacot W, Pantel K, Bartkowiak K, Topart D, Cayrefourcq L, Rossille D, Maudelonde T, Fest T, Alix-Panabieres C
    Mol Oncol, 2015-06-09;9(9):1773-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Disseminated histiocytoses biomarkers beyond BRAFV600E: frequent expression of PD-L1
    Authors: Zoran Gatalica, Nurija Bilalovic, Juan P. Palazzo, Ryan P. Bender, Jeffrey Swensen, Sherri Z. Millis et al.
    Oncotarget
  22. Overexpression of B7-H1 correlates with malignant cell proliferation in pancreatic cancer.
    Authors: Song, Xiao, Liu, Junwei, Lu, Yi, Jin, Hongchua, Huang, Dongshen
    Oncol Rep, 2013-12-31;31(3):1191-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. IFN-gamma generates maturation-arrested dendritic cells that induce T cell hyporesponsiveness independent of Foxp3+ T-regulatory cell generation.
    Authors: Rojas D, Krishnan R
    Immunol. Lett., 2010-05-24;132(1):31-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Fluid nanoporous microinterface enables multiscale-enhanced affinity interaction for tumor-derived extracellular vesicle detection
    Authors: Q Niu, J Gao, K Zhao, X Chen, X Lin, C Huang, Y An, X Xiao, Q Wu, L Cui, P Zhang, L Wu, C Yang
    Proceedings of the National Academy of Sciences of the United States of America, 2022-10-28;119(44):e2213236119.

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Human PD-L1 Antibody
By Anonymous on 11/04/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human PD-L1 Antibody MAB1561
Human PD-L1/B7-H1 Antibody
By Pingshen Hu on 12/16/2017
Application: SPR ASSAY Sample Tested: MCF7 Species: Human
Human PD-L1/B7-H1 Antibody
By Anonymous on 12/11/2017
Application: IHC Sample Tested: Colon cancer tissue Species: Human
Human PD-L1/B7-H1 Antibody
By Anonymous on 12/04/2017
Application: SPR Sample Tested: Human recombinant test antibody Species: Mouse
Other Human PD-L1/B7-H1 Antibody MAB1561
Human PD-L1/B7-H1 Antibody
By Anonymous on 11/20/2017
Application: Flow Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human
Flow Cytometry Human PD-L1/B7-H1 Antibody MAB1561