Human PD-L2/B7-DC Antibody

Name: Human PD-L2/B7-DC Antibody
Brand: R&D Systems
SKU: AF1224
Rating: 5 (1 reviews)

Catalog #: AF1224
7 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1224
AF1224-SP

Detection of Human PD‑L2/B7‑DC by Western Blot.

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All PD-L2/B7-DC Antibodies

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Citations (7)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PD-L2/B7-DC Antibody Summary

Species Reactivity

Human

Specificity

Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PD-L2 is observed and less than 1% cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b and recombinant rat B7-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PD-L2/B7-DC
Leu20-Pro219
Accession # Q9BQ51

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human lung

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) to immobilized Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL) coated at 1 µg/mL (100 µL/well). At 30 µg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PD‑L2/B7‑DC by Western Blot. Western blot shows lysates of human lung tissue and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L2/B7-DC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1224) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Detection of Human PD-L2/B7-DC/PDCD1LG2 by Immunohistochemistry PD-L1, PD-L2, PD-1, CD8, and CD4 expression in p16-positive and p16-negative HNSCC. PD-L1, PD-L2, PD-1, CD8, and CD4 expression was assessed in tumor biopsy tissue from five p16-positive and four p16-negative HNSCC patients using immuno-histochemistry (details in Methods). Representative staining (scale bars, 100 μm) and cumulative data of marker expression (grading scale PD-L1/PD-L2: 1, low; 2, moderate; 3 high expression; grading scale PD-1, CD8, CD4: 1, <50 cells/field; 2, 50–150 cells/field; 3, >150 cells/field). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31379843), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

T cells require a signal induced by the engagement of the T cell receptor and a “co‑stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co‑stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (B7-DC) (1). B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned human PD-L2 cDNA encodes a 273 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 201 aa extracellular region containing one V-like and one C-like Ig domain, a 24 aa transmembrane region, and a 28 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 70% aa sequence identity (2). PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. The other identified ligand is PD-L1. Human PD-L1 and PD-L2 share approximately 41% aa sequence identity and have similar functions. PD-L2 is broadly expressed in tissues. Highest expression was detected by Northern blot analysis in heart, placenta, liver, pancreas, spleen, and lymph node. Lower amounts of expression were observed in lung, smooth muscle, and thymus. Expression of PD-L2 on antigen presenting cell has been examined in detail. Resting B cells, monocytes and dendritic cells do not express PD-L2, expression however can be induced by LPS or BCR activation in B cells, INF-gamma treatment in monocytes, or LPS plus IFN-gamma treatment of dendritic cells. PD-L2 expression is also up regulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co‑stimulatory function for the PD-L2 on resting T cells activated with sub-optimal TCR signals has also been reported (3).

References

Coyle, A.J. and J-C. Gutierrrez-Ramos (2001) Nature Immunol. 2:203.
Latchman Y. et al. (2001) Nature Immun. 2:261.
Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.

Long Name

Programmed Death-1 Ligand-2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Alternate Names

B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PD-L2/B7-DC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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