Home / PDGF-B / Human PDGF-B Antibody

Human PDGF-B Antibody

Catalog #: MAB220
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB220-SP
MAB220-100
MAB220-500
Product Details
Citations (1)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-B Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF-B subunit in direct ELISAs and Western blots. In direct ELISAs, shows approximately 40% cross‑reactivity with recombinant human (rh) PDGF‑AB and no cross-reactivity with rhCTGF, rhLDGF, rhPDGF‑AA, recombinant rat (rr) PDGF‑AA, rrPDGF‑AB, rrPDGF‑BB, rhPDGF‑C, rhPDGF‑D, rhPlGF, rhVEGF, rhVEGF‑C, or rhVEGF‑D.
Source
Monoclonal Mouse IgG1 Clone # 108132
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PDGF-B
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-B

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta.

Long Name
Platelet-derived Growth Factor B
Entrez Gene IDs
5155 (Human); 24628 (Rat); 100070283 (Equine)
Alternate Names
B chain; Becaplermin; C-Sis; FLJ12858; IBGC5; PDGF subunit B; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet-derived growth factor 2; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog); Platelet-derived growth factor beta polypeptide; platelet-derived growth factor subunit B; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis; SIS; SSV

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Citation for Human PDGF-B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

  1. Why are non-reducing conditions recommended for Human PDGF-B Antibody, Catalog # MAB220, in Western blot?

    • Catalog # MAB220 recognizes a conformational epitope on PDGF-B which can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). Reducing conditions will break disulfide bonds and destroy the epitope the antibody recognizes. Therefore, non-reducing conditions are needed for Western Blot.   

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