Human PDGF-BB Antibody

Catalog #: AB-220-NA
5 Citations Datasheet / COA / SDS

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AB-220-NA

Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF‑BB Antibody.

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All PDGF-BB Antibodies

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Citations (5)

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Human PDGF-BB Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF-BB in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human (rh) PDGF-AB is observed, approximately 50% cross-reactivity with recombinant rat PDGF-B and natural porcine PDGF is observed, and less than 10% cross-reactivity with rhPDGF-A is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human PDGF-BB (Catalog # 220-BB)

Neutralization

Measured by its ability to neutralize PDGF‑BB-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND₅₀) is typically 0.25-1.5 µg/mL in the presence of 20 ng/mL Recombinant Human PDGF‑BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF‑BB Antibody. Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-BB (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Polyclonal Antibody (Catalog # AB-220-NA). The ND₅₀ is typically 0.25-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name

Platelet-derived Growth Factor BB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Alternate Names

c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PDGF-BB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
International Journal of Molecular Sciences, 2022-01-14;23(2):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner
Authors: YY Liang, XB Deng, XT Lin, LL Jiang, XT Huang, ZW Mo, YW Yuan, MT Teh
Cell Death Dis, 2020-10-14;11(10):855.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer
Authors: Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al.
Journal of Experimental Medicine
Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation
Authors: Y Zou, S Xu, Y Xiao, Q Qiu, M Shi, J Wang, L Liang, Z Zhan, X Yang, N Olsen, SG Zheng, H Xu
J. Clin. Invest., 2018-09-10;0(0):.
Species: Human
Sample Types: Synovial Fluid
Applications: Neutralization
Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
Authors: Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S
PLoS ONE, 2015-09-18;10(9):e0138587.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization