Human PDGF-C Antibody Summary
Gly230-Gly345
Accession # Q9NRA1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PDGF‑C by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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PDGF‑C in Human Pancreatic Cancer Tissue. PDGF‑C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 µg/mL Goat Anti-Human PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Cell Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody. Recombinant Human PDGF-CC (Catalog # 1687-CC) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND50 is typically 6-24 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF-C
Platelet-derived growth factor C (PDGF-C), also named spinal cord derived growth factor (SCDGF) and follotain, is a member of the PDGF family that binds to the PDGF receptor alpha alpha and alpha beta. PDGF-C is a growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It is a potent mitogen and chemoattractant for cells of mesenchymal origin. It is also required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. In addition, PDGF-C is required for normal skin morphogenesis during embryonic development. PDGF-C plays an important role in angiogenesis and blood vessel development and is involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Western blot analysis of platelets identified 55 kDa and 80 kDa PDGF C forms that were secreted on platelet activation (1).
Product Datasheets
Citations for Human PDGF-C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors
Authors: T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto
Cancer Research, 2023-03-02;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
Oncotarget, 2016-11-22;7(47):77902-77915.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal P
Oncol Rep, 2013-10-10;30(6):2887-96.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Functional Assay, Western Blot -
Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
Atherosclerosis, 2008-05-22;202(2):415-23.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: IHC-Fr, Neutralization, Western Blot
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