Human PDK-1 Antibody

Catalog #: AF864 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF864
AF864-SP

Detection of Human PDK‑1 by Western Blot.

1 Image

All PDK-1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDK-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDK-1 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human PDK-1 synthetic peptide
CKIIKLEYDFPEKFFPKARD
Accession # AAD38505

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human PDK‑1 by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PDK-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF864) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDK-1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDK-1

PDK-1 (Phosphoinositide-dependent Kinase-1), also called PKB kinase and PDPK1, is a member of the AGC group of serine/threonine kinases. Following insulin, growth factor, or pervanadate stimulation, PDK-1 phosphorylates and activates kinases from the Akt, p70 S6K, and RSK families, and is therefore involved in the regulation of cell proliferation and apoptosis.

Long Name

Phosphoinositide Dependent Kinase-1

Entrez Gene IDs

5170 (Human); 18607 (Mouse); 81745 (Rat)

Alternate Names

3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; PDK1; PDK-1; PDPK1; PDPK2; PDPK2P; PkB kinase like gene 1; PKB kinase