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Human PDX-1/IPF1 Biotinylated Antibody

Catalog #: BAF2419
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF2419
PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells.
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Citations (3)
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Human PDX-1/IPF1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDX‑1/IPF1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDX‑1/IPF1
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry PDX-1/IPF1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells using Goat Anti-Human PDX-1/IPF1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDX-1/IPF1

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Long Name
Pancreas/Duodenum Homeobox-1
Entrez Gene IDs
3651 (Human); 18609 (Mouse)
Alternate Names
Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1

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Citations for Human PDX-1/IPF1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A Non-Coding Disease Modifier of Pancreatic Agenesis Identified by Genetic Correction in a Patient-Derived iPSC Line
    Authors: S Kishore, E De Franco, FL Cardenas-D, LR Letourneau, M Sanyoura, C Osorio-Qui, DL French, SAW Greeley, AT Hattersley, P Gadue
    Cell Stem Cell, 2020-05-21;0(0):.
  2. Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background
    Authors: Leavens, KF;Osorio-Quintero, C;Yeuteuh, EA;Perez-Profeta, FT;Dattoli, AA;Cardenas-Diaz, FL;French, DL;Gadue, P;
    Stem cell research
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Flow Cytometry
  3. Severely fibrotic pancreases from young patients with chronic pancreatitis: evidence for a ductal origin of islet neogenesis
    Authors: S. M. Soltani, T. D. O’Brien, G. Loganathan, M. D. Bellin, T. Anazawa, M. Tiwari et al.
    Acta Diabetologica

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