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Human PEDFR/PNPLA2 Antibody

Catalog #: MAB5387
1 Citation Datasheet / COA / SDS
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MAB5387
MAB5387-SP
Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry.
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Human PEDFR/PNPLA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PEDF R/PNPLA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PEDF R is observed.
Source
Monoclonal Mouse IgG2B Clone # 494702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PEDF R/PNPLA2
Val162-Thr332
Accession # Q96AD5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PEDF R/PNPLA2
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PEDF R/PNPLA2 antibody in Y-79 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry. Y-79 human retinoblastoma cell line was stained with Mouse Anti-Human PEDF R/PNPLA2 Monoclonal Antibody (Catalog # MAB5387, filled histogram) or isotype control anti-body (Catalog # MAB0041, open histogram), followed by Phy-coerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PEDFR/PNPLA2

PEDF R (pigment epithelium-derived factor receptor) is also called ATGL (adipose triglyceride lipase), TTS2 (transport-secretion protein 2), iPLA2 zeta (calcium-independent phospholipase A2 zeta) or desnutrin. It is a 54 kDa transmembrane protein that is member 2 of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue, where it catalyzes formation of diacyglycerol from triglyceride. It has also been found in the retina (as a receptor for PEDF), testis, cardiac and skeletal muscle. Human PEDF R shares 85% amino acid (aa) identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.

Long Name
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
Entrez Gene IDs
57104 (Human); 66853 (Mouse); 361676 (Rat)
Alternate Names
Adipose triglyceride lipase; ATGL; ATGL1110001C14Rik; Calcium-independent phospholipase A2; Desnutrin; EC 3.1.1.3; FP17548; IPLA2-zeta; patatin-like phospholipase domain containing 2; patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2-like; PEDF R; PEDFR; PEDF-R; Pigment epithelium-derived factor; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2; TTS-2.2; TTS2DKFZp667M109

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Citation for Human PEDFR/PNPLA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Enhanced efficacy of combination therapy with adenoassociated virus-delivered pigment epithelium-derived factor and cisplatin in a mouse model of Lewis lung carcinoma.
    Authors: He S, Wu Q, Gong C, Luo S, Zhang S, Li M, Lu L, Wei Y, Yang L
    Mol Med Rep, 2014-04-04;9(6):2069-76.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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