Human Pentraxin 2/SAP Antibody Summary
His20-Val233
Accession # P02743
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Pentraxin 2/SAP by Western Blot. Western blot shows lysates of human kidney tissue and human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Pentraxin 2/SAP Monoclonal Antibody (Catalog # MAB1948) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pentraxin 2/SAP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pentraxin 2/SAP
Pentraxin 2 (PTX2), also known as Serum Amyoid P Component (SAP), is a secreted serum glycoprotein that is a universal non-fibrillar component of amyloid deposits. These extracellular deposits of insoluble protein fibrils are the result of protein misfolding and can lead to tissue damage and disease (1, 2). PTX2 belongs to the pentraxin (pentaxin) superfamily, whose members have the characteristic pentagonal discoid arrangement of five non-covalently bound subunits. Pentraxins bind to a variety of molecules in a calcium-dependent lectin-like manner through a pattern-recognition-binding site (1, 4, 5). There are two known subfamilies of pentraxins, the classical or short pentraxin subfamily that includes the serum C-reactive Protein (CRP) and PTX2, and the fusion or long pentraxin subfamily whose members contain pentraxin-related carboxyl-terminal halves (1).
PTX2 and CRP share approximately 50% amino acid sequence identity (2, 5). They are produced and secreted by liver hepatocytes and circulates in plasma. Mouse PTX2 is a major acute-phase protein whose plasma concentrations increase dramatically during an acute phase response (2). In human where CRP is the major acute-phase protein, the plasma concentration of human PTX2 remains relatively constant in response to tissue-damage (2, 5).
PTX2 associates ubiquitously with all amyloid deposits that are implicated in a diverse range of diseases including Alzheimer’s and prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). As a non-fibrillar component, PTX2 regulates the solubility of amyloid fibrils and protects them from degradation by proteolytic enzymes and phagocytic cells. In addition to its role in the pathogenesis of amyloidoses, PTX2 also has an important physiological function in innate immunity (8).
- Goodman, A. et al. (1996) Cytokine Growth Factor Rev. 7:191.
- Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
- Hirschfield, G.M. and P.N. Hawkins (2003) Int. J. Biochem. Cell Biol. 35:1608.
- Emsley, J. et al. (1994) Nature 367:338.
- Mantzouranis, E. et al. (1985) J. Biol. Chem. 260:7752.
- Botto, M. et al. (1997) Nature Medicine 3:855.
- Pepys, M. et al. (2002) Nature 417:254.
- Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.
Product Datasheets
Citation for Human Pentraxin 2/SAP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus
Authors: A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani
Nature Communications, 2021-06-18;12(1):3739.
Species: Human
Sample Types: Plasma
Applications: Western Blot
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